Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Burkholderia phymatum (strain DSM 17167 / STM815)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811Aminoacyl-adenylateUniRule annotation
Binding sitei625 – 6251ATPUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-580-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Bphy_0574
OrganismiBurkholderia phymatum (strain DSM 17167 / STM815)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderia
ProteomesiUP000001192: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Isoleucine--tRNA ligasePRO_1000189139Add
BLAST

Proteomic databases

PRIDEiB2JDY9.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391038.Bphy_0574.

Structurei

3D structure databases

ProteinModelPortaliB2JDY9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionAdd
BLAST
Motifi622 – 6265"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2JDY9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNKKADSKP QARYPVNLLD TPFPMRGDLP RREPQWVKDW QERKIYEKIR
60 70 80 90 100
AASKGRKKFI LHDGPPYANG DIHLGHAVNK ILKDMIVKAR NLAGFDAVYV
110 120 130 140 150
PGWDCHGMPI EIQIEKQFGK SLPAAEVMQK ARAYATEQIE KQKAGFRRLG
160 170 180 190 200
VLGDWDNPYK TMNFANEAGE IRALAKIMEK GYVFRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYKDK TDPTIDVMFT FADPEKTAHA FGLAALPRNE GGIVIWTTTP
260 270 280 290 300
WTIPANQALN LHPEIVYALV DTPRGLLILA EERVEACLKS YGIAGTVIAT
310 320 330 340 350
APGAKLANLR FNHPLASAHP SYKRTSPVYL GDYVTTETGT GIVHSSPAYG
360 370 380 390 400
VEDFISCKTH GMADSDILSP VMGDGRYIES LALFGGLSIW DANPKIVEAL
410 420 430 440 450
DEAGTLLRTE KYLHSYMHCW RHKTPIIYRA TSQWFAGMDI KPNDSDKTLR
460 470 480 490 500
ETALEGIEAT AFYPSWGKQR LFAMIANRPD WTLSRQRQWG VPMAFFVHKE
510 520 530 540 550
TGELHPRTLE LLEEVAKRVE VSGIEAWQTL DPRELIGDDA NMYEKNRDTL
560 570 580 590 600
DVWFDSGTTH WHVLRGSHKD ELQFPADLYL EGSDQHRGWF HSSLLTASML
610 620 630 640 650
DGRPPYNALL THGFTVDGEG RKMSKSLGNG IDPHEVSNRL GAEIIRLWIA
660 670 680 690 700
STDYSGELAI SEEILKRVTE SYRRIRNTLR FLLANLSDFD IGKHARPVGE
710 720 730 740 750
WLEIDRYAVA LTANLQADIL SHYDRYEFHP VVAKLQTFCS EDLGGFYLDV
760 770 780 790 800
LKDRLYTTAA DSKARRSAQT ALYHIAHGLL RLMAPFLSFT AEEAWKVFQP
810 820 830 840 850
QSETIYTETY HAYPDVPEAS TLLDKWTLLR AVRSDVTKAL EEARVANQIG
860 870 880 890 900
SSLQAEVEIR AAGARHDALA SLGADLKFVL ITSGATVVKV DSVDEEGVDV
910 920 930 940
ITSKYLKCER CWHYRKDVGE SAEHPTLCGR CISNLFGNGE TRSAA
Length:945
Mass (Da):105,999
Last modified:June 10, 2008 - v1
Checksum:i0120E0713516D61C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC69765.1.
RefSeqiYP_001856811.1. NC_010622.1.

Genome annotation databases

EnsemblBacteriaiACC69765; ACC69765; Bphy_0574.
GeneIDi6242074.
KEGGibph:Bphy_0574.
PATRICi19187293. VBIBurPhy25146_0602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA. Translation: ACC69765.1.
RefSeqiYP_001856811.1. NC_010622.1.

3D structure databases

ProteinModelPortaliB2JDY9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi391038.Bphy_0574.

Proteomic databases

PRIDEiB2JDY9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC69765; ACC69765; Bphy_0574.
GeneIDi6242074.
KEGGibph:Bphy_0574.
PATRICi19187293. VBIBurPhy25146_0602.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciBPHY391038:GI4Z-580-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 17167 / STM815.

Entry informationi

Entry nameiSYI_BURP8
AccessioniPrimary (citable) accession number: B2JDY9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: January 7, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.