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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei581Aminoacyl-adenylateUniRule annotation1
Binding sitei625ATPUniRule annotation1
Metal bindingi908ZincUniRule annotation1
Metal bindingi911ZincUniRule annotation1
Metal bindingi928ZincUniRule annotation1
Metal bindingi931ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBPHY391038:G1GBS-585-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Bphy_0574
OrganismiParaburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) (Burkholderia phymatum)
Taxonomic identifieri391038 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeParaburkholderia
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891391 – 945Isoleucine--tRNA ligaseAdd BLAST945

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi391038.Bphy_0574

Structurei

3D structure databases

ProteinModelPortaliB2JDY9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi66 – 76"HIGH" regionAdd BLAST11
Motifi622 – 626"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B2JDY9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKKADSKP QARYPVNLLD TPFPMRGDLP RREPQWVKDW QERKIYEKIR
60 70 80 90 100
AASKGRKKFI LHDGPPYANG DIHLGHAVNK ILKDMIVKAR NLAGFDAVYV
110 120 130 140 150
PGWDCHGMPI EIQIEKQFGK SLPAAEVMQK ARAYATEQIE KQKAGFRRLG
160 170 180 190 200
VLGDWDNPYK TMNFANEAGE IRALAKIMEK GYVFRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYKDK TDPTIDVMFT FADPEKTAHA FGLAALPRNE GGIVIWTTTP
260 270 280 290 300
WTIPANQALN LHPEIVYALV DTPRGLLILA EERVEACLKS YGIAGTVIAT
310 320 330 340 350
APGAKLANLR FNHPLASAHP SYKRTSPVYL GDYVTTETGT GIVHSSPAYG
360 370 380 390 400
VEDFISCKTH GMADSDILSP VMGDGRYIES LALFGGLSIW DANPKIVEAL
410 420 430 440 450
DEAGTLLRTE KYLHSYMHCW RHKTPIIYRA TSQWFAGMDI KPNDSDKTLR
460 470 480 490 500
ETALEGIEAT AFYPSWGKQR LFAMIANRPD WTLSRQRQWG VPMAFFVHKE
510 520 530 540 550
TGELHPRTLE LLEEVAKRVE VSGIEAWQTL DPRELIGDDA NMYEKNRDTL
560 570 580 590 600
DVWFDSGTTH WHVLRGSHKD ELQFPADLYL EGSDQHRGWF HSSLLTASML
610 620 630 640 650
DGRPPYNALL THGFTVDGEG RKMSKSLGNG IDPHEVSNRL GAEIIRLWIA
660 670 680 690 700
STDYSGELAI SEEILKRVTE SYRRIRNTLR FLLANLSDFD IGKHARPVGE
710 720 730 740 750
WLEIDRYAVA LTANLQADIL SHYDRYEFHP VVAKLQTFCS EDLGGFYLDV
760 770 780 790 800
LKDRLYTTAA DSKARRSAQT ALYHIAHGLL RLMAPFLSFT AEEAWKVFQP
810 820 830 840 850
QSETIYTETY HAYPDVPEAS TLLDKWTLLR AVRSDVTKAL EEARVANQIG
860 870 880 890 900
SSLQAEVEIR AAGARHDALA SLGADLKFVL ITSGATVVKV DSVDEEGVDV
910 920 930 940
ITSKYLKCER CWHYRKDVGE SAEHPTLCGR CISNLFGNGE TRSAA
Length:945
Mass (Da):105,999
Last modified:June 10, 2008 - v1
Checksum:i0120E0713516D61C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001043 Genomic DNA Translation: ACC69765.1
RefSeqiWP_012399988.1, NC_010622.1

Genome annotation databases

EnsemblBacteriaiACC69765; ACC69765; Bphy_0574
GeneIDi27740246
KEGGibph:Bphy_0574

Similar proteinsi

Entry informationi

Entry nameiSYI_PARP8
AccessioniPrimary (citable) accession number: B2JDY9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: May 23, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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