B2JDW0 (B2JDW0_BURP8) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit 1 HAMAP MF_00444
EC=3.4.21.92 HAMAP MF_00444

Alternative name(s):
Endopeptidase Clp 1 HAMAP MF_00444

Gene names

Name:	clpP1 HAMAP MF_00444
Ordered Locus Names:	Bphy_0544

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family. RuleBase RU003567 HAMAP MF_00444

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00444
Ligand	ATP-binding HAMAP MF_00444 Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease HAMAP MF_00444
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	110	1	By similarity HAMAP MF_00444
	Active site	135	1	By similarity HAMAP MF_00444

Sequences

	Sequence		Length	Mass (Da)
	B2JDW0 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 31992FEBFB604189	FASTA	217	23,634
	10 20 30 40 50 60 MHRHFTSPAN TGFNLVPTVI EQSGRGERAY DIYSRLLRER IVFLVGPVND QSASLIVAQL 70 80 90 100 110 120 LFLESENPDK DISFYINSPG GSVYDGLAIF DTMQFIKPDV STLCTGFAAS MGTFLLAAGA 130 140 150 160 170 180 PGKRYALPNA RIMIHQPSGG GQGTASDVEI HAKEVLYLRE RLNDVLAQRT GQSIAQIEKD 190 200 210 TDRDNFMSAE AAKTYGLVDD VLTTRVPLPV DALHGTT

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001043 Genomic DNA. Translation: ACC69736.1.
RefSeq	YP_001856782.1. NC_010622.1.
3D structure databases
ProteinModelPortal	B2JDW0.
SMR	B2JDW0. Positions 15-205.
ModBase	Search...
Protein-protein interaction databases
STRING	B2JDW0.
Protein family/group databases
MEROPS	S14.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6242045.
GenomeReviews	Gene locus Bphy_0544 in contig CP001043_GR.
KEGG	bph:Bphy_0544.
PATRIC	19187231. VBIBurPhy25146_0571.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	QHSRIMI.
ProtClustDB	CLSK2314407.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR023562. Pept_S14/S49. IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
KO	K01358.
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. ClpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B2JDW0_BURP8

Accession

Primary (citable) accession number: B2JDW0

Entry history

Integrated into UniProtKB/TrEMBL:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)