Reviewed, UniProtKB/Swiss-Prot B2JD61 (ACSA_BURP8)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase
    Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	Bphy_1938

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 660	660	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000137261
Sites
	Active site	530	1	By similarity
Amino acid modifications
	Modified residue	625	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2JD61-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: BDC68F538B23A5BF	FASTA	660	72,379
	10 20 30 40 50 60 MSAIESVLQE RRVFQPSAEV AAQATVSGMD AYKALVAEAE RDYEGFWGRL ARETLSWNKP 70 80 90 100 110 120 FTKVLDESNA PFYKWYDDGE LNASYNSIDR HVEAGNGERV AIIFEADDGT ITNVTYNDLL 130 140 150 160 170 180 QRVSRFANAL KQRGIKKGDR VVIYMPMSVE GIVAMQACAR IGATHSVVFG GFSSKSLNER 190 200 210 220 230 240 LVDVGAVALV TSDEQMRGGK ALPLKNIADE ALAMGGCEAV KSVIVYQRTG GKIGWDDKRD 250 260 270 280 290 300 LWMHEITASE SDHCPPEWVG AEHPLFILYT SGSTGKPKGV QHSTGGYLLW AAQTMKWTFD 310 320 330 340 350 360 WKPTDVFWCT ADIGWVTGHS YITYGPLTLG GTQVVFEGVP TYPNAGRFWD MIQKHKVTVF 370 380 390 400 410 420 YTAPTAIRSL IKAAEADPKV HPKSYDLSTL RIIGTVGEPI NPEAWVWYYE NVGGSRCPIV 430 440 450 460 470 480 DTWWQTETGG HMITPLPGAT PLVPGSCTLP LPGIMAAVVD ETGQDVPNGQ GGILVVKRPW 490 500 510 520 530 540 PSMIRTIWGD PERYKKSYFP EELGGTLYLA GDGSVRDKET GYFTIMGRID DVLNVSGHRL 550 560 570 580 590 600 GTMEIESALV ANPLVAEAAV VGRPDDTTGE AVCAFVVLKR ARPEGEEAAK IAADLRNWVG 610 620 630 640 650 660 KEIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEEI TQDVSTLENP AILDQLGESR

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001043 Genomic DNA. Translation: ACC71117.1.
RefSeq	YP_001858163.1.
3D structure databases
SMR	B2JD61. Positions 22-659.
ModBase	Search...
Genome annotation databases
GeneID	6243427.
GenomeReviews	Gene locus Bphy_1938 in contig CP001043_GR.
KEGG	bph:Bphy_1938.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	ETASEHC.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_BURP8

Accession

Primary (citable) accession number: B2JD61

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	February 9, 2010
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)