Reviewed, UniProtKB/Swiss-Prot B2JCJ1 (HUTI_BURP8)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	Bphy_1810

Organism

Burkholderia phymatum (strain DSM 17167 / STM815) [Complete proteome] [HAMAP]

Taxonomic identifier

391038 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity.
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Imidazolonepropionase HAMAP MF_00372		PRO_1000121539
Sites
	Metal binding	67	1	Zinc or iron By similarity
	Metal binding	69	1	Zinc or iron By similarity
	Metal binding	237	1	Zinc or iron By similarity
	Metal binding	312	1	Zinc or iron By similarity
	Binding site	76	1	Substrate By similarity
	Binding site	89	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	172	1	Substrate By similarity
	Binding site	240	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2JCJ1-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 5DA4A8F14A9AD2F6	FASTA	406	43,750
	10 20 30 40 50 60 MKQTVWNHLK LCPQGDPAQT IDDAAIAVEN GRIVWLGAVR ELPAQYAAWP REDLGGAWVT 70 80 90 100 110 120 PGLVDCHTHL VYGGQRADEF AQRLAGVSYE EIAKQGGGIV STVRATRAAD EASLFAQSAA 130 140 150 160 170 180 RLEAMLAEGV TAIEIKSGYG LDLQSERKML RVARQLGERY PVKVYTTFLG AHALPPEFAS 190 200 210 220 230 240 RADDYIDEVC EGMLPALADE GLVDAVDVFC ERIGFSLEQS ERVFNAAARH GLPVKMHAEQ 250 260 270 280 290 300 LSNAGGTALA ARYGALSADH LEFLDEAGVA AMKEAGTVAV LLPGAYYFIR ETQLPPVELL 310 320 330 340 350 360 RRYGVPIAIS TDSNPGTSPA TSLPLMMNMA TTLFRMTVPE VLRGVTCHAA QALGKADTHG 370 380 390 400 TLAVGRAADF AVWSVQSLAE LAYWIGRPLC ERVVRGGETV HLRHAR

References

[1]

"Complete sequence of chromosome 1 of Burkholderia phymatum STM815." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Bruce D., Goodwin L., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Mikhailova N., Bacher J., Blanchard J., Cohan F., James E., Lawrence J., Lizotte-Waniewski M., Moulin L., Rainey P., Riley M., Souza V., Wertz J., Young P. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001043 Genomic DNA. Translation: ACC70992.1.
RefSeq	YP_001858038.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6243301.
GenomeReviews	Gene locus Bphy_1810 in contig CP001043_GR.
KEGG	bph:Bphy_1810.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MNMACTL.
Family and domain databases
HAMAP	MF_00372. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. [Graphical view]
Pfam	PF01979. Amidohydro_1. 2 hits. [Graphical view]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BURP8

Accession

Primary (citable) accession number: B2JCJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)