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B2J7T9 (SYL_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Npun_F4156
OrganismNostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]
Taxonomic identifier63737 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length871 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 871871Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091339

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif634 – 6385"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6371ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2J7T9 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: F57D19EF68173BAF

FASTA87198,573
        10         20         30         40         50         60 
MDSRYNPAAI EEKRQKTWLE LGLDKTPSAS NKPKFYALSM FPYPSGSLHM GHVRNYTITD 

        70         80         90        100        110        120 
VIARFKRMQG YRVIHPMGWD AFGLPAENAA IDRGVPPAKW TYQNITQMRQ QLQRLGLSID 

       130        140        150        160        170        180 
WECELATCSP DYYKWTQWIF LQFLQAGLAY QKEAAVNWDP IDQTVLANEQ VDNEGRSWRS 

       190        200        210        220        230        240 
GAIVERKLLR QWFFKITDYA EELLNDLDKL TGWPERVKLM QANWIGKSTG AYLEFPIVGI 

       250        260        270        280        290        300 
DEKIAVYTTR PDTVYGVSYL VLAPEHPLTN RVTTKEQQAA VEVFIKEVSN QSELERTSED 

       310        320        330        340        350        360 
KPKRGIPTGG VAINPFTGEE VPIWIADYVL YEYGTGAVMG VPAHDVRDFK FAKNYDLPIN 

       370        380        390        400        410        420 
FVIASPDDVA GFDLTPTSEI DGITQLVEVD YKQAYTEPGI LINSGAFTGI SSTDAKQAII 

       430        440        450        460        470        480 
EYAEKQDFGK VRVQYRLRDW LISRQRYWGA PIPVIHCPNC GIVPVPDKDL PVQLPEEVEF 

       490        500        510        520        530        540 
TGRCGSPLTQ LESWVNVPCP TCGTPAKRET DTMDTFIDSS WYFLRFPDAK NEQQVFDSSK 

       550        560        570        580        590        600 
VNDWMPVNQY VGGIEHAILH LLYSRFFTKV LRDRGLLNFD EPFQRLLTQG MVQGLTYLNP 

       610        620        630        640        650        660 
NKGGKDKWIP SNLVNSADPR DPQTDEPLQR LYATMSKSKG NGVAPEDVIS KYGIDTARMF 

       670        680        690        700        710        720 
ILFKAPPEKD LEWDEADVEG QFRFLNRVWR LVTDYITAGV SRKKAQSDLT KAEKELRRAI 

       730        740        750        760        770        780 
HTAIQAVTED VEDEYQFNTA ISELMKLSNA LSDADKNSPI YAEGIRTLVI LIAPFAPHIA 

       790        800        810        820        830        840 
DELWHLLGES DSIHTQTWPS FDPAALVADE ITLVIQVMGK TRGAIQVPAQ ADKAALEKYA 

       850        860        870 
RESEIAQRYI EGKEIKKVIV VPGKLVNFVV S 

« Hide

References

[1]"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. expand/collapse author list , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001037 Genomic DNA. Translation: ACC82534.1.
RefSeqYP_001867477.1. NC_010628.1.

3D structure databases

ProteinModelPortalB2J7T9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING63737.Npun_F4156.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACC82534; ACC82534; Npun_F4156.
GeneID6253501.
KEGGnpu:Npun_F4156.
PATRIC22761434. VBINosPun48114_4876.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycNPUN63737:GJNP-4025-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_NOSP7
AccessionPrimary (citable) accession number: B2J7T9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: April 16, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries