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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei637 – 6371ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNPUN63737:GJNP-4025-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Npun_F4156
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000001191: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 871871Leucine--tRNA ligasePRO_1000091339Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi63737.Npun_F4156.

Structurei

3D structure databases

ProteinModelPortaliB2J7T9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi634 – 6385"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiB2J7T9.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2J7T9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSRYNPAAI EEKRQKTWLE LGLDKTPSAS NKPKFYALSM FPYPSGSLHM
60 70 80 90 100
GHVRNYTITD VIARFKRMQG YRVIHPMGWD AFGLPAENAA IDRGVPPAKW
110 120 130 140 150
TYQNITQMRQ QLQRLGLSID WECELATCSP DYYKWTQWIF LQFLQAGLAY
160 170 180 190 200
QKEAAVNWDP IDQTVLANEQ VDNEGRSWRS GAIVERKLLR QWFFKITDYA
210 220 230 240 250
EELLNDLDKL TGWPERVKLM QANWIGKSTG AYLEFPIVGI DEKIAVYTTR
260 270 280 290 300
PDTVYGVSYL VLAPEHPLTN RVTTKEQQAA VEVFIKEVSN QSELERTSED
310 320 330 340 350
KPKRGIPTGG VAINPFTGEE VPIWIADYVL YEYGTGAVMG VPAHDVRDFK
360 370 380 390 400
FAKNYDLPIN FVIASPDDVA GFDLTPTSEI DGITQLVEVD YKQAYTEPGI
410 420 430 440 450
LINSGAFTGI SSTDAKQAII EYAEKQDFGK VRVQYRLRDW LISRQRYWGA
460 470 480 490 500
PIPVIHCPNC GIVPVPDKDL PVQLPEEVEF TGRCGSPLTQ LESWVNVPCP
510 520 530 540 550
TCGTPAKRET DTMDTFIDSS WYFLRFPDAK NEQQVFDSSK VNDWMPVNQY
560 570 580 590 600
VGGIEHAILH LLYSRFFTKV LRDRGLLNFD EPFQRLLTQG MVQGLTYLNP
610 620 630 640 650
NKGGKDKWIP SNLVNSADPR DPQTDEPLQR LYATMSKSKG NGVAPEDVIS
660 670 680 690 700
KYGIDTARMF ILFKAPPEKD LEWDEADVEG QFRFLNRVWR LVTDYITAGV
710 720 730 740 750
SRKKAQSDLT KAEKELRRAI HTAIQAVTED VEDEYQFNTA ISELMKLSNA
760 770 780 790 800
LSDADKNSPI YAEGIRTLVI LIAPFAPHIA DELWHLLGES DSIHTQTWPS
810 820 830 840 850
FDPAALVADE ITLVIQVMGK TRGAIQVPAQ ADKAALEKYA RESEIAQRYI
860 870
EGKEIKKVIV VPGKLVNFVV S
Length:871
Mass (Da):98,573
Last modified:June 10, 2008 - v1
Checksum:iF57D19EF68173BAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC82534.1.
RefSeqiYP_001867477.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC82534; ACC82534; Npun_F4156.
GeneIDi6253501.
KEGGinpu:Npun_F4156.
PATRICi22761434. VBINosPun48114_4876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC82534.1.
RefSeqiYP_001867477.1. NC_010628.1.

3D structure databases

ProteinModelPortaliB2J7T9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi63737.Npun_F4156.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC82534; ACC82534; Npun_F4156.
GeneIDi6253501.
KEGGinpu:Npun_F4156.
PATRICi22761434. VBINosPun48114_4876.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.
PhylomeDBiB2J7T9.

Enzyme and pathway databases

BioCyciNPUN63737:GJNP-4025-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29133 / PCC 73102.

Entry informationi

Entry nameiSYL_NOSP7
AccessioniPrimary (citable) accession number: B2J7T9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: January 7, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.