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Protein

Argininosuccinate synthase

Gene

argG

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Binding sitei89 – 891CitrullineUniRule annotation
Binding sitei119 – 1191ATP; via amide nitrogenUniRule annotation
Binding sitei121 – 1211AspartateUniRule annotation
Binding sitei125 – 1251AspartateUniRule annotation
Binding sitei125 – 1251CitrullineUniRule annotation
Binding sitei126 – 1261AspartateUniRule annotation
Binding sitei129 – 1291CitrullineUniRule annotation
Binding sitei177 – 1771CitrullineUniRule annotation
Binding sitei186 – 1861CitrullineUniRule annotation
Binding sitei262 – 2621CitrullineUniRule annotation
Binding sitei274 – 2741CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNPUN63737:GJNP-5427-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Npun_R5563
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000001191 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Argininosuccinate synthasePRO_1000089046Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi63737.Npun_R5563.

Structurei

3D structure databases

ProteinModelPortaliB2J6U2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.
PhylomeDBiB2J6U2.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2J6U2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRAKKVVLA YSGGVDTSVC IPYLKQEWGV EEVITLAADL GQGDELEPIR
60 70 80 90 100
EKALKSGASE SLVADVKDSF VKDYAFGAIQ ANALYENRYP LGTALARPLI
110 120 130 140 150
AKILVETAEK YGADAIAHGC TGKGNDQVRF DVSVTALNPN LKILAPAREW
160 170 180 190 200
GMSREETIAY GEKFGIPSPV KKSSPYSIDK NLLGRSIEAG LLEDPSFEPP
210 220 230 240 250
EEIYEMTKAI ADTPNEPEYI KIGFHGGIPT TLNGIAKQPV ELIEELNQLV
260 270 280 290 300
GNHGVGRIDM IENRLVGIKS REIYESPAML VLIQAHRDLE SLTLTADVTH
310 320 330 340 350
YKRGIEETYS QIVYNGLWYS PLKVALDAFI QKTQERVSGT VRVKLFKGNS
360 370 380 390 400
TIVGRSSDSS LYTPDLATYG ADDQFDHKAA EGFIYVWGLP TRIWSRQIKS
Length:400
Mass (Da):43,988
Last modified:June 10, 2008 - v1
Checksum:iDDB2F0140011588E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC83868.1.
RefSeqiWP_012411812.1. NC_010628.1.
YP_001868811.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC83868; ACC83868; Npun_R5563.
KEGGinpu:Npun_R5563.
PATRICi22764700. VBINosPun48114_6485.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC83868.1.
RefSeqiWP_012411812.1. NC_010628.1.
YP_001868811.1. NC_010628.1.

3D structure databases

ProteinModelPortaliB2J6U2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi63737.Npun_R5563.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC83868; ACC83868; Npun_R5563.
KEGGinpu:Npun_R5563.
PATRICi22764700. VBINosPun48114_6485.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiPAREWGM.
OrthoDBiEOG6K9QCV.
PhylomeDBiB2J6U2.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciNPUN63737:GJNP-5427-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29133 / PCC 73102.

Entry informationi

Entry nameiASSY_NOSP7
AccessioniPrimary (citable) accession number: B2J6U2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: May 27, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.