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B2J4Z5

- B2J4Z5_NOSP7

UniProt

B2J4Z5 - B2J4Z5_NOSP7

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Protein
Submitted name:

Uncharacterized protein

Gene

Npun_R2028

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Unreviewed - Annotation score: 1 out of 5- Protein predictedi

Functioni

Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.SAAS annotation

Catalytic activityi

1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O2 = 3-(methylthio)propanoate + formate + CO.SAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Note: Binds 1 iron ion per monomer.SAAS annotation
  • Note: Binds 1 nickel ion per monomer.SAAS annotation

GO - Molecular functioni

  1. acireductone dioxygenase [iron(II)-requiring] activity Source: InterPro
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

IronSAAS annotation, Metal-bindingSAAS annotation, NickelSAAS annotation

Enzyme and pathway databases

BioCyciNPUN63737:GJNP-1972-MONOMER.

Names & Taxonomyi

Protein namesi
Submitted name:
Uncharacterized proteinImported
Gene namesi
Ordered Locus Names:Npun_R2028Imported
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)Imported
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000001191: Chromosome

Interactioni

Subunit structurei

Monomer.SAAS annotation

Protein-protein interaction databases

STRINGi63737.Npun_R2028.

Structurei

3D structure databases

ProteinModelPortaliB2J4Z5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiCOG1791.
HOGENOMiHOG000201072.
KOiK08967.
OMAiYGRYHTH.
OrthoDBiEOG6ZPT06.
PhylomeDBiB2J4Z5.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR004313. Acireductn_dOase_family.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF03079. ARD. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

B2J4Z5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANLLLDDGT IESDLGEIAR ELAPFGIQLR HYNPGTSLLF PNLLSQDVLT
60 70 80 90 100
ESEKHYCVEL HNSVFEFLQQ ENGAIWCDLL NVHPGSFNLH HLIATYGRYH
110 120 130 140 150
THPAPEPLYV LAGEMIYGFV RPDGSQVQLL VQAQDYLYIP ADVEHWCSPT
160 170 180
ASLNFKAVRY FAIAEGWVPN YTGTQVSDSL NKPG
Length:184
Mass (Da):20,645
Last modified:June 10, 2008 - v1
Checksum:iF9A2C2586A41DAF0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC80655.1.
RefSeqiYP_001865598.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC80655; ACC80655; Npun_R2028.
GeneIDi6251448.
KEGGinpu:Npun_R2028.
PATRICi22756282. VBINosPun48114_2333.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC80655.1 .
RefSeqi YP_001865598.1. NC_010628.1.

3D structure databases

ProteinModelPortali B2J4Z5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 63737.Npun_R2028.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACC80655 ; ACC80655 ; Npun_R2028 .
GeneIDi 6251448.
KEGGi npu:Npun_R2028.
PATRICi 22756282. VBINosPun48114_2333.

Phylogenomic databases

eggNOGi COG1791.
HOGENOMi HOG000201072.
KOi K08967.
OMAi YGRYHTH.
OrthoDBi EOG6ZPT06.
PhylomeDBi B2J4Z5.

Enzyme and pathway databases

BioCyci NPUN63737:GJNP-1972-MONOMER.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
InterProi IPR004313. Acireductn_dOase_family.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
Pfami PF03079. ARD. 1 hit.
[Graphical view ]
SUPFAMi SSF51182. SSF51182. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29133 / PCC 73102Imported.

Entry informationi

Entry nameiB2J4Z5_NOSP7
AccessioniPrimary (citable) accession number: B2J4Z5
Entry historyi
Integrated into UniProtKB/TrEMBL: June 10, 2008
Last sequence update: June 10, 2008
Last modified: November 26, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3