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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi9MagnesiumUniRule annotation1
Metal bindingi76MagnesiumUniRule annotation1
Metal bindingi148MagnesiumUniRule annotation1
Metal bindingi151MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:Npun_R5350
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000001191 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000905411 – 163Crossover junction endodeoxyribonuclease RuvCAdd BLAST163

Interactioni

Protein-protein interaction databases

STRINGi63737.Npun_R5350.

Structurei

3D structure databases

ProteinModelPortaliB2J4H5.
SMRiB2J4H5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105NHU. Bacteria.
COG0817. LUCA.
HOGENOMiHOG000012182.
KOiK01159.
OMAiFFNRNVT.
OrthoDBiPOG091H0202.
PhylomeDBiB2J4H5.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.

Sequencei

Sequence statusi: Complete.

B2J4H5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKRILGLDP GLATLGFGVI TCTQIANKVP ETTVNMLDFG VIKTSADVEM
60 70 80 90 100
GLRLCTLFDD LHTVMEEFQP DLVAIEKLFF YRMSSTILVA QARGVLILVL
110 120 130 140 150
GQRRLPYVEF TPAQIKQALT GYGNADKLDV QEAVARELDL DEIPKPDDAA
160
DALAVALTAS YQL
Length:163
Mass (Da):17,875
Last modified:June 10, 2008 - v1
Checksum:i0F1A8247A73C27A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC83665.1.
RefSeqiWP_012411616.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC83665; ACC83665; Npun_R5350.
KEGGinpu:Npun_R5350.
PATRICi22764204. VBINosPun48114_6237.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC83665.1.
RefSeqiWP_012411616.1. NC_010628.1.

3D structure databases

ProteinModelPortaliB2J4H5.
SMRiB2J4H5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi63737.Npun_R5350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC83665; ACC83665; Npun_R5350.
KEGGinpu:Npun_R5350.
PATRICi22764204. VBINosPun48114_6237.

Phylogenomic databases

eggNOGiENOG4105NHU. Bacteria.
COG0817. LUCA.
HOGENOMiHOG000012182.
KOiK01159.
OMAiFFNRNVT.
OrthoDBiPOG091H0202.
PhylomeDBiB2J4H5.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC. 1 hit.
InterProiIPR012337. RNaseH-like_dom.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRUVC_NOSP7
AccessioniPrimary (citable) accession number: B2J4H5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: November 2, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.