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B2J4H5

- RUVC_NOSP7

UniProt

B2J4H5 - RUVC_NOSP7

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi9 – 91MagnesiumUniRule annotation
    Metal bindingi76 – 761MagnesiumUniRule annotation
    Metal bindingi148 – 1481MagnesiumUniRule annotation
    Metal bindingi151 – 1511MagnesiumUniRule annotation

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciNPUN63737:GJNP-5220-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
    Alternative name(s):
    Holliday junction nuclease RuvCUniRule annotation
    Holliday junction resolvase RuvCUniRule annotation
    Gene namesi
    Name:ruvCUniRule annotation
    Ordered Locus Names:Npun_R5350
    OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
    Taxonomic identifieri63737 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000001191: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 163163Crossover junction endodeoxyribonuclease RuvCPRO_1000090541Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi63737.Npun_R5350.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012182.
    KOiK01159.
    OMAiYHPDEMA.
    OrthoDBiEOG6RG044.
    PhylomeDBiB2J4H5.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B2J4H5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKRILGLDP GLATLGFGVI TCTQIANKVP ETTVNMLDFG VIKTSADVEM    50
    GLRLCTLFDD LHTVMEEFQP DLVAIEKLFF YRMSSTILVA QARGVLILVL 100
    GQRRLPYVEF TPAQIKQALT GYGNADKLDV QEAVARELDL DEIPKPDDAA 150
    DALAVALTAS YQL 163
    Length:163
    Mass (Da):17,875
    Last modified:June 10, 2008 - v1
    Checksum:i0F1A8247A73C27A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001037 Genomic DNA. Translation: ACC83665.1.
    RefSeqiYP_001868608.1. NC_010628.1.

    Genome annotation databases

    EnsemblBacteriaiACC83665; ACC83665; Npun_R5350.
    GeneIDi6254696.
    KEGGinpu:Npun_R5350.
    PATRICi22764204. VBINosPun48114_6237.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001037 Genomic DNA. Translation: ACC83665.1 .
    RefSeqi YP_001868608.1. NC_010628.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 63737.Npun_R5350.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACC83665 ; ACC83665 ; Npun_R5350 .
    GeneIDi 6254696.
    KEGGi npu:Npun_R5350.
    PATRICi 22764204. VBINosPun48114_6237.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012182.
    KOi K01159.
    OMAi YHPDEMA.
    OrthoDBi EOG6RG044.
    PhylomeDBi B2J4H5.

    Enzyme and pathway databases

    BioCyci NPUN63737:GJNP-5220-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29133 / PCC 73102.

    Entry informationi

    Entry nameiRUVC_NOSP7
    AccessioniPrimary (citable) accession number: B2J4H5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3