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B2J3M9

- LEUC_NOSP7

UniProt

B2J3M9 - LEUC_NOSP7

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Protein
3-isopropylmalate dehydratase large subunit
Gene
leuC, Npun_F0162
Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S) By similarity
Metal bindingi407 – 4071Iron-sulfur (4Fe-4S) By similarity
Metal bindingi410 – 4101Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciNPUN63737:GJNP-161-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunit (EC:4.2.1.33)
Alternative name(s):
Alpha-IPM isomerase
Short name:
IPMI
Isopropylmalate isomerase
Gene namesi
Name:leuC
Ordered Locus Names:Npun_F0162
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000001191: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4674673-isopropylmalate dehydratase large subunitUniRule annotation
PRO_1000135698Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi63737.Npun_F0162.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiIDKHLIH.
OrthoDBiEOG600DP5.
PhylomeDBiB2J3M9.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2J3M9-1 [UniParc]FASTAAdd to Basket

« Hide

MSKGTLFDKV WDLHTVGTLP SGLTQLFIGL HLIHEVTSPQ AFAMLRERGL    50
KVLFPERTVA TVDHIVPTEN QARPFVDSLA EEMIQALENN CQENNITFYN 100
IGSGSQGIVH VIAPELGLTQ PGMTIACGDS HTSSHGAFGA IAFGIGTSQV 150
RDVLASQTLA LSKLKVRKIE VNGNLNPGVY AKDVILHIIR TLGVKGGVGY 200
GYEFAGTTFE QMNMEERMTV CNMAIEGGAR CGYVNPDRVT YDYLKGRDFA 250
PKGADWDKAV AWWDSIKSDV DAQYDDVVVF DAAEISPTVT WGITPGQGIG 300
VNQSVPQPEE LLEEDRFIAE EAYRYMDLYP GQPIKGTKID VCFIGSCTNG 350
RISDLREAAK IAKGRHVAEG IKAFVVPGSE RVKQEAETEG LDKIFQEAGF 400
EWREPGCSMC LAMNPDKLQG RQISASSSNR NFKGRQGSSS GRTLLMSPAM 450
VATAAIKGEV SDVRELL 467
Length:467
Mass (Da):50,845
Last modified:June 10, 2008 - v1
Checksum:i37399D152AF56677
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001037 Genomic DNA. Translation: ACC78959.1.
RefSeqiYP_001863902.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC78959; ACC78959; Npun_F0162.
GeneIDi6249620.
KEGGinpu:Npun_F0162.
PATRICi22751933. VBINosPun48114_0184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001037 Genomic DNA. Translation: ACC78959.1 .
RefSeqi YP_001863902.1. NC_010628.1.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 63737.Npun_F0162.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACC78959 ; ACC78959 ; Npun_F0162 .
GeneIDi 6249620.
KEGGi npu:Npun_F0162.
PATRICi 22751933. VBINosPun48114_0184.

Phylogenomic databases

eggNOGi COG0065.
HOGENOMi HOG000226972.
KOi K01703.
OMAi IDKHLIH.
OrthoDBi EOG600DP5.
PhylomeDBi B2J3M9.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci NPUN63737:GJNP-161-MONOMER.

Family and domain databases

Gene3Di 3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPi MF_01026. LeuC_type1.
InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR00170. leuC. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29133 / PCC 73102.

Entry informationi

Entry nameiLEUC_NOSP7
AccessioniPrimary (citable) accession number: B2J3M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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