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B2J3M9 (LEUC_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydratase large subunit

EC=4.2.1.33
Alternative name(s):
Alpha-IPM isomerase
Short name=IPMI
Isopropylmalate isomerase
Gene names
Name:leuC
Ordered Locus Names:Npun_F0162
OrganismNostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]
Taxonomic identifier63737 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length467 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate By similarity. HAMAP-Rule MF_01026

Catalytic activity

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate. HAMAP-Rule MF_01026

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_01026

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4. HAMAP-Rule MF_01026

Subunit structure

Heterodimer of LeuC and LeuD By similarity. HAMAP-Rule MF_01026

Sequence similarities

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4674673-isopropylmalate dehydratase large subunit HAMAP-Rule MF_01026
PRO_1000135698

Sites

Metal binding3471Iron-sulfur (4Fe-4S) By similarity
Metal binding4071Iron-sulfur (4Fe-4S) By similarity
Metal binding4101Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
B2J3M9 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 37399D152AF56677

FASTA46750,845
        10         20         30         40         50         60 
MSKGTLFDKV WDLHTVGTLP SGLTQLFIGL HLIHEVTSPQ AFAMLRERGL KVLFPERTVA 

        70         80         90        100        110        120 
TVDHIVPTEN QARPFVDSLA EEMIQALENN CQENNITFYN IGSGSQGIVH VIAPELGLTQ 

       130        140        150        160        170        180 
PGMTIACGDS HTSSHGAFGA IAFGIGTSQV RDVLASQTLA LSKLKVRKIE VNGNLNPGVY 

       190        200        210        220        230        240 
AKDVILHIIR TLGVKGGVGY GYEFAGTTFE QMNMEERMTV CNMAIEGGAR CGYVNPDRVT 

       250        260        270        280        290        300 
YDYLKGRDFA PKGADWDKAV AWWDSIKSDV DAQYDDVVVF DAAEISPTVT WGITPGQGIG 

       310        320        330        340        350        360 
VNQSVPQPEE LLEEDRFIAE EAYRYMDLYP GQPIKGTKID VCFIGSCTNG RISDLREAAK 

       370        380        390        400        410        420 
IAKGRHVAEG IKAFVVPGSE RVKQEAETEG LDKIFQEAGF EWREPGCSMC LAMNPDKLQG 

       430        440        450        460 
RQISASSSNR NFKGRQGSSS GRTLLMSPAM VATAAIKGEV SDVRELL 

« Hide

References

[1]"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. expand/collapse author list , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001037 Genomic DNA. Translation: ACC78959.1.
RefSeqYP_001863902.1. NC_010628.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING63737.Npun_F0162.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACC78959; ACC78959; Npun_F0162.
GeneID6249620.
KEGGnpu:Npun_F0162.
PATRIC22751933. VBINosPun48114_0184.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0065.
HOGENOMHOG000226972.
KOK01703.
OMAIDKHLIH.
OrthoDBEOG600DP5.
PhylomeDBB2J3M9.

Enzyme and pathway databases

BioCycNPUN63737:GJNP-161-MONOMER.
UniPathwayUPA00048; UER00071.

Family and domain databases

Gene3D3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPMF_01026. LeuC_type1.
InterProIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERPTHR11670. PTHR11670. 1 hit.
PfamPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSPR00415. ACONITASE.
SUPFAMSSF53732. SSF53732. 1 hit.
TIGRFAMsTIGR00170. leuC. 1 hit.
PROSITEPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEUC_NOSP7
AccessionPrimary (citable) accession number: B2J3M9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways