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B2J3M9

- LEUC_NOSP7

UniProt

B2J3M9 - LEUC_NOSP7

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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi407 – 4071Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi410 – 4101Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciNPUN63737:GJNP-161-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:Npun_F0162
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000001191: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4674673-isopropylmalate dehydratase large subunitPRO_1000135698Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi63737.Npun_F0162.

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiIDKHLIH.
OrthoDBiEOG600DP5.
PhylomeDBiB2J3M9.

Family and domain databases

Gene3Di3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2J3M9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKGTLFDKV WDLHTVGTLP SGLTQLFIGL HLIHEVTSPQ AFAMLRERGL
60 70 80 90 100
KVLFPERTVA TVDHIVPTEN QARPFVDSLA EEMIQALENN CQENNITFYN
110 120 130 140 150
IGSGSQGIVH VIAPELGLTQ PGMTIACGDS HTSSHGAFGA IAFGIGTSQV
160 170 180 190 200
RDVLASQTLA LSKLKVRKIE VNGNLNPGVY AKDVILHIIR TLGVKGGVGY
210 220 230 240 250
GYEFAGTTFE QMNMEERMTV CNMAIEGGAR CGYVNPDRVT YDYLKGRDFA
260 270 280 290 300
PKGADWDKAV AWWDSIKSDV DAQYDDVVVF DAAEISPTVT WGITPGQGIG
310 320 330 340 350
VNQSVPQPEE LLEEDRFIAE EAYRYMDLYP GQPIKGTKID VCFIGSCTNG
360 370 380 390 400
RISDLREAAK IAKGRHVAEG IKAFVVPGSE RVKQEAETEG LDKIFQEAGF
410 420 430 440 450
EWREPGCSMC LAMNPDKLQG RQISASSSNR NFKGRQGSSS GRTLLMSPAM
460
VATAAIKGEV SDVRELL
Length:467
Mass (Da):50,845
Last modified:June 10, 2008 - v1
Checksum:i37399D152AF56677
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC78959.1.
RefSeqiYP_001863902.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC78959; ACC78959; Npun_F0162.
GeneIDi6249620.
KEGGinpu:Npun_F0162.
PATRICi22751933. VBINosPun48114_0184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC78959.1 .
RefSeqi YP_001863902.1. NC_010628.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 63737.Npun_F0162.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACC78959 ; ACC78959 ; Npun_F0162 .
GeneIDi 6249620.
KEGGi npu:Npun_F0162.
PATRICi 22751933. VBINosPun48114_0184.

Phylogenomic databases

eggNOGi COG0065.
HOGENOMi HOG000226972.
KOi K01703.
OMAi IDKHLIH.
OrthoDBi EOG600DP5.
PhylomeDBi B2J3M9.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci NPUN63737:GJNP-161-MONOMER.

Family and domain databases

Gene3Di 3.30.499.10. 2 hits.
3.40.1060.10. 1 hit.
HAMAPi MF_01026. LeuC_type1.
InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR00170. leuC. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29133 / PCC 73102.

Entry informationi

Entry nameiLEUC_NOSP7
AccessioniPrimary (citable) accession number: B2J3M9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: November 26, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3