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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: L-leucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. 2-isopropylmalate synthase (leuA), 2-isopropylmalate synthase (leuA)
  2. 3-isopropylmalate dehydratase large subunit (leuC), 3-isopropylmalate dehydratase small subunit (Npun_F0163)
  3. 3-isopropylmalate dehydrogenase (leuB)
  4. no protein annotated in this organism
This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi347Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi407Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi410Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciNPUN63737:G1GBR-168-MONOMER
UniPathwayiUPA00048; UER00071

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:Npun_F0162
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000001191 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001356981 – 4673-isopropylmalate dehydratase large subunitAdd BLAST467

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi63737.Npun_F0162

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQI Bacteria
COG0065 LUCA
HOGENOMiHOG000226972
KOiK01703
OMAiFDHQVPA
OrthoDBiPOG091H02GD
PhylomeDBiB2J3M9

Family and domain databases

CDDicd01583 IPMI, 1 hit
Gene3Di3.30.499.10, 1 hit
HAMAPiMF_01026 LeuC_type1, 1 hit
InterProiView protein in InterPro
IPR004430 3-IsopropMal_deHydase_lsu
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR033941 IPMI_cat
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR00170 leuC, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

B2J3M9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKGTLFDKV WDLHTVGTLP SGLTQLFIGL HLIHEVTSPQ AFAMLRERGL
60 70 80 90 100
KVLFPERTVA TVDHIVPTEN QARPFVDSLA EEMIQALENN CQENNITFYN
110 120 130 140 150
IGSGSQGIVH VIAPELGLTQ PGMTIACGDS HTSSHGAFGA IAFGIGTSQV
160 170 180 190 200
RDVLASQTLA LSKLKVRKIE VNGNLNPGVY AKDVILHIIR TLGVKGGVGY
210 220 230 240 250
GYEFAGTTFE QMNMEERMTV CNMAIEGGAR CGYVNPDRVT YDYLKGRDFA
260 270 280 290 300
PKGADWDKAV AWWDSIKSDV DAQYDDVVVF DAAEISPTVT WGITPGQGIG
310 320 330 340 350
VNQSVPQPEE LLEEDRFIAE EAYRYMDLYP GQPIKGTKID VCFIGSCTNG
360 370 380 390 400
RISDLREAAK IAKGRHVAEG IKAFVVPGSE RVKQEAETEG LDKIFQEAGF
410 420 430 440 450
EWREPGCSMC LAMNPDKLQG RQISASSSNR NFKGRQGSSS GRTLLMSPAM
460
VATAAIKGEV SDVRELL
Length:467
Mass (Da):50,845
Last modified:June 10, 2008 - v1
Checksum:i37399D152AF56677
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA Translation: ACC78959.1
RefSeqiWP_012406988.1, NC_010628.1

Genome annotation databases

EnsemblBacteriaiACC78959; ACC78959; Npun_F0162
KEGGinpu:Npun_F0162

Similar proteinsi

Entry informationi

Entry nameiLEUC_NOSP7
AccessioniPrimary (citable) accession number: B2J3M9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: March 28, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health