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B2J3M9

- LEUC_NOSP7

UniProt

B2J3M9 - LEUC_NOSP7

Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

    Catalytic activityi

    (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi347 – 3471Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi407 – 4071Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi410 – 4101Iron-sulfur (4Fe-4S)UniRule annotation

    GO - Molecular functioni

    1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. leucine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciNPUN63737:GJNP-161-MONOMER.
    UniPathwayiUPA00048; UER00071.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
    Alternative name(s):
    Alpha-IPM isomeraseUniRule annotation
    Short name:
    IPMIUniRule annotation
    Isopropylmalate isomeraseUniRule annotation
    Gene namesi
    Name:leuCUniRule annotation
    Ordered Locus Names:Npun_F0162
    OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
    Taxonomic identifieri63737 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000001191: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 4674673-isopropylmalate dehydratase large subunitPRO_1000135698Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of LeuC and LeuD.UniRule annotation

    Protein-protein interaction databases

    STRINGi63737.Npun_F0162.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0065.
    HOGENOMiHOG000226972.
    KOiK01703.
    OMAiIDKHLIH.
    OrthoDBiEOG600DP5.
    PhylomeDBiB2J3M9.

    Family and domain databases

    Gene3Di3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPiMF_01026. LeuC_type1.
    InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view]
    PANTHERiPTHR11670. PTHR11670. 1 hit.
    PfamiPF00330. Aconitase. 1 hit.
    [Graphical view]
    PRINTSiPR00415. ACONITASE.
    SUPFAMiSSF53732. SSF53732. 1 hit.
    TIGRFAMsiTIGR00170. leuC. 1 hit.
    PROSITEiPS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B2J3M9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKGTLFDKV WDLHTVGTLP SGLTQLFIGL HLIHEVTSPQ AFAMLRERGL    50
    KVLFPERTVA TVDHIVPTEN QARPFVDSLA EEMIQALENN CQENNITFYN 100
    IGSGSQGIVH VIAPELGLTQ PGMTIACGDS HTSSHGAFGA IAFGIGTSQV 150
    RDVLASQTLA LSKLKVRKIE VNGNLNPGVY AKDVILHIIR TLGVKGGVGY 200
    GYEFAGTTFE QMNMEERMTV CNMAIEGGAR CGYVNPDRVT YDYLKGRDFA 250
    PKGADWDKAV AWWDSIKSDV DAQYDDVVVF DAAEISPTVT WGITPGQGIG 300
    VNQSVPQPEE LLEEDRFIAE EAYRYMDLYP GQPIKGTKID VCFIGSCTNG 350
    RISDLREAAK IAKGRHVAEG IKAFVVPGSE RVKQEAETEG LDKIFQEAGF 400
    EWREPGCSMC LAMNPDKLQG RQISASSSNR NFKGRQGSSS GRTLLMSPAM 450
    VATAAIKGEV SDVRELL 467
    Length:467
    Mass (Da):50,845
    Last modified:June 10, 2008 - v1
    Checksum:i37399D152AF56677
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001037 Genomic DNA. Translation: ACC78959.1.
    RefSeqiYP_001863902.1. NC_010628.1.

    Genome annotation databases

    EnsemblBacteriaiACC78959; ACC78959; Npun_F0162.
    GeneIDi6249620.
    KEGGinpu:Npun_F0162.
    PATRICi22751933. VBINosPun48114_0184.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001037 Genomic DNA. Translation: ACC78959.1 .
    RefSeqi YP_001863902.1. NC_010628.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 63737.Npun_F0162.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACC78959 ; ACC78959 ; Npun_F0162 .
    GeneIDi 6249620.
    KEGGi npu:Npun_F0162.
    PATRICi 22751933. VBINosPun48114_0184.

    Phylogenomic databases

    eggNOGi COG0065.
    HOGENOMi HOG000226972.
    KOi K01703.
    OMAi IDKHLIH.
    OrthoDBi EOG600DP5.
    PhylomeDBi B2J3M9.

    Enzyme and pathway databases

    UniPathwayi UPA00048 ; UER00071 .
    BioCyci NPUN63737:GJNP-161-MONOMER.

    Family and domain databases

    Gene3Di 3.30.499.10. 2 hits.
    3.40.1060.10. 1 hit.
    HAMAPi MF_01026. LeuC_type1.
    InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
    IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
    IPR015937. Acoase/IPM_deHydtase.
    IPR001030. Acoase/IPM_deHydtase_lsu_aba.
    IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
    IPR018136. Aconitase_4Fe-4S_BS.
    [Graphical view ]
    PANTHERi PTHR11670. PTHR11670. 1 hit.
    Pfami PF00330. Aconitase. 1 hit.
    [Graphical view ]
    PRINTSi PR00415. ACONITASE.
    SUPFAMi SSF53732. SSF53732. 1 hit.
    TIGRFAMsi TIGR00170. leuC. 1 hit.
    PROSITEi PS00450. ACONITASE_1. 1 hit.
    PS01244. ACONITASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29133 / PCC 73102.

    Entry informationi

    Entry nameiLEUC_NOSP7
    AccessioniPrimary (citable) accession number: B2J3M9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3