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B2J1D2 (ISPD_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT
Gene names
Name:ispD
Ordered Locus Names:Npun_F5020
OrganismNostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]
Taxonomic identifier63737 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length227 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108

Sequence similarities

Belongs to the IspD family.

Ontologies

Keywords
   Biological processIsoprene biosynthesis
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processisoprenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2272272-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108
PRO_1000094335

Sites

Site131Transition state stabilizer By similarity
Site201Transition state stabilizer By similarity
Site1521Positions MEP for the nucleophilic attack By similarity
Site2081Positions MEP for the nucleophilic attack By similarity

Sequences

Sequence LengthMass (Da)Tools
B2J1D2 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 86FE25054FAFB9F7

FASTA22724,799
        10         20         30         40         50         60 
MYLLIPAAGI GKRMGSNRNK LLLKVRSQPI IAWTLLAAEA ANTISWIGII SQPTDWPDFR 

        70         80         90        100        110        120 
AILADLKLTK PVELIQGGST RQESVYNGLQ ALPLAAEQVL IHDGARCLAT PDLFNSCAQA 

       130        140        150        160        170        180 
IRHCPGLIAG VPVKDTIKVV DEQGIIQETP DRQKLWAAQT PQGFDVKLLK QCHAEGVRQG 

       190        200        210        220 
WEVTDDAALF EKCGIEVRIV EGEETNLKVT TPQDLAIAEF ILTTRGV

« Hide

References

[1]"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. expand/collapse author list , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001037 Genomic DNA. Translation: ACC83363.1.
RefSeqYP_001868306.1. NC_010628.1.

3D structure databases

ProteinModelPortalB2J1D2.
ModBaseSearch...

Protein-protein interaction databases

STRINGB2J1D2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6254375.
GenomeReviewsGene locus Npun_F5020 in contig CP001037_GR.
KEGGnpu:Npun_F5020.
PATRIC22763440. VBINosPun48114_5860.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG672839.
OMASKVIVVC.
PhylomeDBB2J1D2.
ProtClustDBPRK00155.

Family and domain databases

HAMAPMF_00108. IspD.
[Tree]
InterProIPR001228. ISPD_synthase.
IPR018294. ISPD_synthase_CS.
[Graphical view]
KOK00991.
PfamPF01128. IspD. 1 hit.
[Graphical view]
TIGRFAMsTIGR00453. IspD. 1 hit.
PROSITEPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPD_NOSP7
AccessionPrimary (citable) accession number: B2J1D2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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