B2J0V5 (SYGA_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	Npun_R1642

Organism

Nostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]

Taxonomic identifier

63737 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000101210

Sequences

	Sequence		Length	Mass (Da)
	B2J0V5 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 7EFD10C5C2FAE23C	FASTA	312	35,756
	10 20 30 40 50 60 MNFQSVISLL HHFWGTRGCL IAQPYDIEKG AGTKNPQTFL RALGPEPWAV AYVEPCRRPT 70 80 90 100 110 120 DGRYGENPNR FQHYYQYQVL IKPSPDNIQE IYLDSLRALG IRPEDHDIRF VEDNWEDATV 130 140 150 160 170 180 GAWGTGWEVW LDGMEITQFT YFQQCGGIDC RPVSIEITYG LERLTMYLQQ VEAITKIHWT 190 200 210 220 230 240 DNITYGDVFL QNEIEQSTYN FEASNPELLL TLFSLYEQEA TQLTKKGLVL PSLDYVMKCS 250 260 270 280 290 300 HTFNLLDARG VISVTERTRY IARIRHLARK VAHLYVEQRE KLGFPLLKDL KPAIPVGQVE 310 VAATQTKSAS AG

References

[1]

"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001037 Genomic DNA. Translation: ACC80322.1.
RefSeq	YP_001865265.1. NC_010628.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2J0V5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6251081.
GenomeReviews	Gene locus Npun_R1642 in contig CP001037_GR.
KEGG	npu:Npun_R1642.
PATRIC	22755365. VBINosPun48114_1882.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG285316.
OMA	CRRPTDG.
PhylomeDB	B2J0V5.
ProtClustDB	PRK09348.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_NOSP7

Accession

Primary (citable) accession number: B2J0V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program