B2J0Q5 (RNPA_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease P protein component
Short name=RNase P protein
Short name=RNaseP protein
EC=3.1.26.5

Alternative name(s):
Protein C5

Gene names

Name:	rnpA
Ordered Locus Names:	Npun_R1587

Organism

Nostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]

Taxonomic identifier

63737 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc › Nostoc punctiforme

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme By similarity. HAMAP-Rule MF_00227
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00227
Subunit structure	Consists of a catalytic RNA component (M1 or RnpB) and a protein subunit By similarity.
Sequence similarities	Belongs to the RnpA family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA 5'-leader removal Inferred from electronic annotation. Source: HAMAP
Molecular_function	ribonuclease P activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 140	140	Ribonuclease P protein component HAMAP-Rule MF_00227		PRO_1000100377

Sequences

	Sequence		Length	Mass (Da)
	B2J0Q5 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 871099C5639BA710	FASTA	140	15,735
	10 20 30 40 50 60 MALPKANRLK SRKDFQAVFR EGIRRNGSYL TLRALKPLYS RKPSLDTATQ TTQPIESVHI 70 80 90 100 110 120 SSIRIGISIS TKVSKRAVVR NRIKRQITSA LYSLLPRLAP GWRLVFIVKP TAAESKCVSP 130 140 QFLQELEQLL AQAEVFDGNS

References

[1]

"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001037 Genomic DNA. Translation: ACC80272.1.
RefSeq	YP_001865215.1. NC_010628.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	63737.Npun_R1587.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACC80272; ACC80272; Npun_R1587.
GeneID	6251028.
KEGG	npu:Npun_R1587.
PATRIC	22755233. VBINosPun48114_1816.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0594.
HOGENOM	HOG000266301.
KO	K03536.
OMA	YEHFLRE.
ProtClustDB	PRK03031.
Enzyme and pathway databases
BioCyc	NPUN63737:GJNP-1552-MONOMER.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00227. RNase_P.
InterPro	IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR000100. RNase_P. [Graphical view]
Pfam	PF00825. Ribonuclease_P. 1 hit. [Graphical view]
ProDom	PD003629. Ribonuclease_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00188. rnpA. 1 hit.
PROSITE	PS00648. RIBONUCLEASE_P. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPA_NOSP7

Accession

Primary (citable) accession number: B2J0Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program