Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B2J0J6 (DDL_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Npun_R6628
OrganismNostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]
Taxonomic identifier63737 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000091198

Regions

Domain148 – 357210ATP-grasp
Nucleotide binding184 – 23956ATP By similarity

Sites

Metal binding3101Magnesium or manganese 1 By similarity
Metal binding3241Magnesium or manganese 1 By similarity
Metal binding3241Magnesium or manganese 2 By similarity
Metal binding3261Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B2J0J6 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 99CDCD36BC83FAF9

FASTA36639,781
        10         20         30         40         50         60 
MTKLRVGLLF GGRSGEHEVS IKSARAIAKA LSVDENASKY EILPFYIQKD GRWLAGEAPQ 

        70         80         90        100        110        120 
KVLETGSPLL EAEQSTSEGN LTSNPQVQTL SKWESPSQVA QVDIWFPVLH GPNGEDGTIQ 

       130        140        150        160        170        180 
GLLTLMQVPF VGSGVLGSAM GMDKIAMKMT FEQAGLAQVK YKAITRAQVW SNPCVFPKLC 

       190        200        210        220        230        240 
DEIEAALGYP AFVKPANLGS SVGIAKVRSR QELEAALDNA ASYDRRLVVE AGVVAREVEC 

       250        260        270        280        290        300 
AVLGNDQPQA SVVGEISYDS DFYDYETKYT EDRADLLIPA PIPDAIARQI QDMALQAFAA 

       310        320        330        340        350        360 
VDAAGLARVD FFYVEATQEV LINEINTLPG FTATSMYPQL WAHSGVSFPE LVDRLIQLAL 


ERYSPS 

« Hide

References

[1]"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. expand/collapse author list , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001037 Genomic DNA. Translation: ACC84886.1.
RefSeqYP_001869829.1. NC_010628.1.

3D structure databases

ProteinModelPortalB2J0J6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING63737.Npun_R6628.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACC84886; ACC84886; Npun_R6628.
GeneID6255972.
KEGGnpu:Npun_R6628.
PATRIC22767321. VBINosPun48114_7778.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMARSAYGIY.
OrthoDBEOG64BQ73.
PhylomeDBB2J0J6.

Enzyme and pathway databases

BioCycNPUN63737:GJNP-6495-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_NOSP7
AccessionPrimary (citable) accession number: B2J0J6
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways