B2J0A5 (ISPE_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Npun_R4911

Organism

Nostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]

Taxonomic identifier

63737 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092101
Regions
	Nucleotide binding	99 – 109	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	141	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2J0A5 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 8AADA1E7E796388F	FASTA	317	34,494
	10 20 30 40 50 60 MRSYSLIAPA KINLYLEIIG DRPDGYHELA MILQSIGLSD QIDVRSISTD SIRVHCNHPQ 70 80 90 100 110 120 VPTDKSNLAY RAAELMVRQF PEAFAKYGGV EITVNKQIPV AAGLAGGSTN AAAVLVGIDL 130 140 150 160 170 180 LWKLGLTQSE LEELGGTLGS DVPFCVAGGT AIATGRGEQL SPLPSLDNIY IVLGKYRSLE 190 200 210 220 230 240 VSTPWAYKTY RQQFGHSYII DTDNLAARAS AVHSGAIVKA ILHKDTREIA QKLHNDLERV 250 260 270 280 290 300 VLPAYPQVLH LREVFANQEG VLGTMMSGSG PTVFALFESQ QQAELVLQQV REAIIDEDLE 310 LFVTRTITHG IKVTSSV

References

[1]

"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001037 Genomic DNA. Translation: ACC83257.1.
RefSeq	YP_001868200.1. NC_010628.1.
3D structure databases
ProteinModelPortal	B2J0A5.
ModBase	Search...
Protein-protein interaction databases
STRING	B2J0A5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6254262.
GenomeReviews	Gene locus Npun_R4911 in contig CP001037_GR.
KEGG	npu:Npun_R4911.
PATRIC	22763186. VBINosPun48114_5738.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	QLCFGRG.
PhylomeDB	B2J0A5.
ProtClustDB	PRK02534.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_NOSP7

Accession

Primary (citable) accession number: B2J0A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program