SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B2IZP5

- SYI_NOSP7

UniProt

B2IZP5 - SYI_NOSP7

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, Npun_F1478
Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei571 – 5711Aminoacyl-adenylate By similarity
Binding sitei615 – 6151ATP By similarity
Metal bindingi928 – 9281Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity
Metal bindingi948 – 9481Zinc By similarity
Metal bindingi951 – 9511Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNPUN63737:GJNP-1449-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Npun_F1478
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000001191: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189185Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi63737.Npun_F1478.

Structurei

3D structure databases

ProteinModelPortaliB2IZP5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionUniRule annotation
Add
BLAST
Motifi612 – 6165"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.
PhylomeDBiB2IZP5.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2IZP5-1 [UniParc]FASTAAdd to Basket

« Hide

MTESGSYKDT VNLPKTSFDM RANAIKREPE IQKFWEENKI YDRLFENNPG    50
ELFILHDGPP YANGSLHIGH ALNKILKDII NRYQMLRGRK VRYVPGWDCH 100
GLPIELKVLQ NMKSAERQNL TPLQLRQKAK EFGLATVDNQ RQNFKRYGIW 150
GDWDNPYLTL KPEYEAAQIG VFGQMFLKGY IYRGLKPVHW SPSSKTALAE 200
AELEYPEGHV SRSIYAAFPV TSLAEAVKPL LAEYQSDLGV AIWTTTPWTI 250
PGNLAVAVNA DLNYAVVEVS QSEAQSKFKY LIVAADLVER LSSTLGLELT 300
LKATFKGNDL EHTTYRHPLF DRESPIVVGG DYITTESGTG LVHTAPGHGQ 350
EDYIVGQRYG LPILAPVDDN GNFTQEAGEF AGLNVLGDGN QAVIDALAAA 400
GSLLKEEPYP HKYPYDWRTK KPTIFRATEQ WFASVEGFRE EALKAIATVK 450
WIPAQGENRI TPMVAERSDW CISRQRSWGV PIPVFYDEAT GEPLLNEEII 500
NHVQGIIAEK GSDAWWELSV EELLPESYRQ NGKSYRRGTD TMDVWFDSGS 550
SWASVVQQRP ELRYPADIYL EGSDQHRGWF QSSLLTSVAV NDIAPYKTVL 600
THGFALDEQG RKMSKSEGNV VDPNTIIEGG KNQKVEPAYG ADVLRLWVSS 650
VDYSGDVRIG KNIIKQMNDV RGKIRNTARF LLGSLDDFDP EKDTVPFEEL 700
PELDRYMLHR ITEVFEEVTE AFESFQFFRF FQTVQNFCVV DLSNFYLDVA 750
KDRLYISAKD AFRRRSCQTV LKIALDNLAR AIAPVLSHTA EDIWQYLPYK 800
TPYKSVFEAG WVQVEEKWRN PELAEFWSEL RKIRDKVNKT LEIYRLQKTI 850
GSSLEAKVVV HVFDERLRQR LQALNPNTHG SVEKSNGVDE LRYLFLASQV 900
EVPSEPIFEE GDDAGIYIQV YKADGEKCDR CWNYSTHVGE SAEHPLICER 950
CVAALAGEF 959
Length:959
Mass (Da):108,907
Last modified:June 10, 2008 - v1
Checksum:i489BC403D056D89B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001037 Genomic DNA. Translation: ACC80175.1.
RefSeqiYP_001865118.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC80175; ACC80175; Npun_F1478.
GeneIDi6250925.
KEGGinpu:Npun_F1478.
PATRICi22754981. VBINosPun48114_1691.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001037 Genomic DNA. Translation: ACC80175.1 .
RefSeqi YP_001865118.1. NC_010628.1.

3D structure databases

ProteinModelPortali B2IZP5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 63737.Npun_F1478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACC80175 ; ACC80175 ; Npun_F1478 .
GeneIDi 6250925.
KEGGi npu:Npun_F1478.
PATRICi 22754981. VBINosPun48114_1691.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.
PhylomeDBi B2IZP5.

Enzyme and pathway databases

BioCyci NPUN63737:GJNP-1449-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29133 / PCC 73102.

Entry informationi

Entry nameiSYI_NOSP7
AccessioniPrimary (citable) accession number: B2IZP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi