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B2IZP5 (SYI_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:Npun_F1478
OrganismNostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]
Taxonomic identifier63737 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length959 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 959959Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_1000189185

Regions

Motif60 – 7011"HIGH" region HAMAP-Rule MF_02002
Motif612 – 6165"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding9281Zinc By similarity
Metal binding9311Zinc By similarity
Metal binding9481Zinc By similarity
Metal binding9511Zinc By similarity
Binding site5711Aminoacyl-adenylate By similarity
Binding site6151ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B2IZP5 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 489BC403D056D89B

FASTA959108,907
        10         20         30         40         50         60 
MTESGSYKDT VNLPKTSFDM RANAIKREPE IQKFWEENKI YDRLFENNPG ELFILHDGPP 

        70         80         90        100        110        120 
YANGSLHIGH ALNKILKDII NRYQMLRGRK VRYVPGWDCH GLPIELKVLQ NMKSAERQNL 

       130        140        150        160        170        180 
TPLQLRQKAK EFGLATVDNQ RQNFKRYGIW GDWDNPYLTL KPEYEAAQIG VFGQMFLKGY 

       190        200        210        220        230        240 
IYRGLKPVHW SPSSKTALAE AELEYPEGHV SRSIYAAFPV TSLAEAVKPL LAEYQSDLGV 

       250        260        270        280        290        300 
AIWTTTPWTI PGNLAVAVNA DLNYAVVEVS QSEAQSKFKY LIVAADLVER LSSTLGLELT 

       310        320        330        340        350        360 
LKATFKGNDL EHTTYRHPLF DRESPIVVGG DYITTESGTG LVHTAPGHGQ EDYIVGQRYG 

       370        380        390        400        410        420 
LPILAPVDDN GNFTQEAGEF AGLNVLGDGN QAVIDALAAA GSLLKEEPYP HKYPYDWRTK 

       430        440        450        460        470        480 
KPTIFRATEQ WFASVEGFRE EALKAIATVK WIPAQGENRI TPMVAERSDW CISRQRSWGV 

       490        500        510        520        530        540 
PIPVFYDEAT GEPLLNEEII NHVQGIIAEK GSDAWWELSV EELLPESYRQ NGKSYRRGTD 

       550        560        570        580        590        600 
TMDVWFDSGS SWASVVQQRP ELRYPADIYL EGSDQHRGWF QSSLLTSVAV NDIAPYKTVL 

       610        620        630        640        650        660 
THGFALDEQG RKMSKSEGNV VDPNTIIEGG KNQKVEPAYG ADVLRLWVSS VDYSGDVRIG 

       670        680        690        700        710        720 
KNIIKQMNDV RGKIRNTARF LLGSLDDFDP EKDTVPFEEL PELDRYMLHR ITEVFEEVTE 

       730        740        750        760        770        780 
AFESFQFFRF FQTVQNFCVV DLSNFYLDVA KDRLYISAKD AFRRRSCQTV LKIALDNLAR 

       790        800        810        820        830        840 
AIAPVLSHTA EDIWQYLPYK TPYKSVFEAG WVQVEEKWRN PELAEFWSEL RKIRDKVNKT 

       850        860        870        880        890        900 
LEIYRLQKTI GSSLEAKVVV HVFDERLRQR LQALNPNTHG SVEKSNGVDE LRYLFLASQV 

       910        920        930        940        950 
EVPSEPIFEE GDDAGIYIQV YKADGEKCDR CWNYSTHVGE SAEHPLICER CVAALAGEF 

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References

[1]"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. expand/collapse author list , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001037 Genomic DNA. Translation: ACC80175.1.
RefSeqYP_001865118.1. NC_010628.1.

3D structure databases

ProteinModelPortalB2IZP5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING63737.Npun_F1478.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACC80175; ACC80175; Npun_F1478.
GeneID6250925.
KEGGnpu:Npun_F1478.
PATRIC22754981. VBINosPun48114_1691.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAVLGDWDN.
OrthoDBEOG644ZM1.
ProtClustDBPRK05743.

Enzyme and pathway databases

BioCycNPUN63737:GJNP-1449-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_NOSP7
AccessionPrimary (citable) accession number: B2IZP5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: February 19, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries