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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei571Aminoacyl-adenylateUniRule annotation1
Binding sitei615ATPUniRule annotation1
Metal bindingi928ZincUniRule annotation1
Metal bindingi931ZincUniRule annotation1
Metal bindingi948ZincUniRule annotation1
Metal bindingi951ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNPUN63737:G1GBR-1515-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Npun_F1478
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000001191 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891851 – 959Isoleucine--tRNA ligaseAdd BLAST959

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi63737.Npun_F1478

Structurei

3D structure databases

ProteinModelPortaliB2IZP5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi60 – 70"HIGH" regionAdd BLAST11
Motifi612 – 616"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I
PhylomeDBiB2IZP5

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B2IZP5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESGSYKDT VNLPKTSFDM RANAIKREPE IQKFWEENKI YDRLFENNPG
60 70 80 90 100
ELFILHDGPP YANGSLHIGH ALNKILKDII NRYQMLRGRK VRYVPGWDCH
110 120 130 140 150
GLPIELKVLQ NMKSAERQNL TPLQLRQKAK EFGLATVDNQ RQNFKRYGIW
160 170 180 190 200
GDWDNPYLTL KPEYEAAQIG VFGQMFLKGY IYRGLKPVHW SPSSKTALAE
210 220 230 240 250
AELEYPEGHV SRSIYAAFPV TSLAEAVKPL LAEYQSDLGV AIWTTTPWTI
260 270 280 290 300
PGNLAVAVNA DLNYAVVEVS QSEAQSKFKY LIVAADLVER LSSTLGLELT
310 320 330 340 350
LKATFKGNDL EHTTYRHPLF DRESPIVVGG DYITTESGTG LVHTAPGHGQ
360 370 380 390 400
EDYIVGQRYG LPILAPVDDN GNFTQEAGEF AGLNVLGDGN QAVIDALAAA
410 420 430 440 450
GSLLKEEPYP HKYPYDWRTK KPTIFRATEQ WFASVEGFRE EALKAIATVK
460 470 480 490 500
WIPAQGENRI TPMVAERSDW CISRQRSWGV PIPVFYDEAT GEPLLNEEII
510 520 530 540 550
NHVQGIIAEK GSDAWWELSV EELLPESYRQ NGKSYRRGTD TMDVWFDSGS
560 570 580 590 600
SWASVVQQRP ELRYPADIYL EGSDQHRGWF QSSLLTSVAV NDIAPYKTVL
610 620 630 640 650
THGFALDEQG RKMSKSEGNV VDPNTIIEGG KNQKVEPAYG ADVLRLWVSS
660 670 680 690 700
VDYSGDVRIG KNIIKQMNDV RGKIRNTARF LLGSLDDFDP EKDTVPFEEL
710 720 730 740 750
PELDRYMLHR ITEVFEEVTE AFESFQFFRF FQTVQNFCVV DLSNFYLDVA
760 770 780 790 800
KDRLYISAKD AFRRRSCQTV LKIALDNLAR AIAPVLSHTA EDIWQYLPYK
810 820 830 840 850
TPYKSVFEAG WVQVEEKWRN PELAEFWSEL RKIRDKVNKT LEIYRLQKTI
860 870 880 890 900
GSSLEAKVVV HVFDERLRQR LQALNPNTHG SVEKSNGVDE LRYLFLASQV
910 920 930 940 950
EVPSEPIFEE GDDAGIYIQV YKADGEKCDR CWNYSTHVGE SAEHPLICER

CVAALAGEF
Length:959
Mass (Da):108,907
Last modified:June 10, 2008 - v1
Checksum:i489BC403D056D89B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA Translation: ACC80175.1
RefSeqiWP_012408194.1, NC_010628.1

Genome annotation databases

EnsemblBacteriaiACC80175; ACC80175; Npun_F1478
KEGGinpu:Npun_F1478

Similar proteinsi

Entry informationi

Entry nameiSYI_NOSP7
AccessioniPrimary (citable) accession number: B2IZP5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: March 28, 2018
This is version 70 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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