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B2IZP5

- SYI_NOSP7

UniProt

B2IZP5 - SYI_NOSP7

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei571 – 5711Aminoacyl-adenylateUniRule annotation
Binding sitei615 – 6151ATPUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation
Metal bindingi948 – 9481ZincUniRule annotation
Metal bindingi951 – 9511ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciNPUN63737:GJNP-1449-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Npun_F1478
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000001191: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 959959Isoleucine--tRNA ligasePRO_1000189185Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi63737.Npun_F1478.

Structurei

3D structure databases

ProteinModelPortaliB2IZP5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi60 – 7011"HIGH" regionAdd
BLAST
Motifi612 – 6165"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.
PhylomeDBiB2IZP5.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2IZP5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTESGSYKDT VNLPKTSFDM RANAIKREPE IQKFWEENKI YDRLFENNPG
60 70 80 90 100
ELFILHDGPP YANGSLHIGH ALNKILKDII NRYQMLRGRK VRYVPGWDCH
110 120 130 140 150
GLPIELKVLQ NMKSAERQNL TPLQLRQKAK EFGLATVDNQ RQNFKRYGIW
160 170 180 190 200
GDWDNPYLTL KPEYEAAQIG VFGQMFLKGY IYRGLKPVHW SPSSKTALAE
210 220 230 240 250
AELEYPEGHV SRSIYAAFPV TSLAEAVKPL LAEYQSDLGV AIWTTTPWTI
260 270 280 290 300
PGNLAVAVNA DLNYAVVEVS QSEAQSKFKY LIVAADLVER LSSTLGLELT
310 320 330 340 350
LKATFKGNDL EHTTYRHPLF DRESPIVVGG DYITTESGTG LVHTAPGHGQ
360 370 380 390 400
EDYIVGQRYG LPILAPVDDN GNFTQEAGEF AGLNVLGDGN QAVIDALAAA
410 420 430 440 450
GSLLKEEPYP HKYPYDWRTK KPTIFRATEQ WFASVEGFRE EALKAIATVK
460 470 480 490 500
WIPAQGENRI TPMVAERSDW CISRQRSWGV PIPVFYDEAT GEPLLNEEII
510 520 530 540 550
NHVQGIIAEK GSDAWWELSV EELLPESYRQ NGKSYRRGTD TMDVWFDSGS
560 570 580 590 600
SWASVVQQRP ELRYPADIYL EGSDQHRGWF QSSLLTSVAV NDIAPYKTVL
610 620 630 640 650
THGFALDEQG RKMSKSEGNV VDPNTIIEGG KNQKVEPAYG ADVLRLWVSS
660 670 680 690 700
VDYSGDVRIG KNIIKQMNDV RGKIRNTARF LLGSLDDFDP EKDTVPFEEL
710 720 730 740 750
PELDRYMLHR ITEVFEEVTE AFESFQFFRF FQTVQNFCVV DLSNFYLDVA
760 770 780 790 800
KDRLYISAKD AFRRRSCQTV LKIALDNLAR AIAPVLSHTA EDIWQYLPYK
810 820 830 840 850
TPYKSVFEAG WVQVEEKWRN PELAEFWSEL RKIRDKVNKT LEIYRLQKTI
860 870 880 890 900
GSSLEAKVVV HVFDERLRQR LQALNPNTHG SVEKSNGVDE LRYLFLASQV
910 920 930 940 950
EVPSEPIFEE GDDAGIYIQV YKADGEKCDR CWNYSTHVGE SAEHPLICER

CVAALAGEF
Length:959
Mass (Da):108,907
Last modified:June 10, 2008 - v1
Checksum:i489BC403D056D89B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001037 Genomic DNA. Translation: ACC80175.1.
RefSeqiYP_001865118.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC80175; ACC80175; Npun_F1478.
GeneIDi6250925.
KEGGinpu:Npun_F1478.
PATRICi22754981. VBINosPun48114_1691.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001037 Genomic DNA. Translation: ACC80175.1 .
RefSeqi YP_001865118.1. NC_010628.1.

3D structure databases

ProteinModelPortali B2IZP5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 63737.Npun_F1478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACC80175 ; ACC80175 ; Npun_F1478 .
GeneIDi 6250925.
KEGGi npu:Npun_F1478.
PATRICi 22754981. VBINosPun48114_1691.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.
PhylomeDBi B2IZP5.

Enzyme and pathway databases

BioCyci NPUN63737:GJNP-1449-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29133 / PCC 73102.

Entry informationi

Entry nameiSYI_NOSP7
AccessioniPrimary (citable) accession number: B2IZP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3