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B2IX54 (PUR9_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:purH
Ordered Locus Names:Npun_R6316
OrganismNostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]
Taxonomic identifier63737 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaNostocalesNostocaceaeNostoc

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139

Sequence similarities

Belongs to the PurH family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139
PRO_1000096077

Sequences

Sequence LengthMass (Da)Tools
B2IX54 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: D25BFB75E9D5654D

FASTA51454,873
        10         20         30         40         50         60 
MARLALLSVS NKTGIIDLAR SLVEEFDFDL ISSGGTAQAL KDAGLPVTKV ADYTGSPEIL 

        70         80         90        100        110        120 
GGRVKTLHPR IHGGILARRD VPQDITDLEN NQIRPIDLVV VNLYPFEETI AKPGVTLLEA 

       130        140        150        160        170        180 
VEQIDIGGPA MLRASSKNFA HLAVLCDPAQ YDEYLEELRQ NNGVASLEFR QKAALKGFSH 

       190        200        210        220        230        240 
TASYDQAIAQ ALTCQFASYL ADTQQYTLSG TQLQSLRYGE NPHQPATWYQ TGTTPTGWAA 

       250        260        270        280        290        300 
ATKLQGKELS YNNLVDLEAA RRIIAEFTDT PAATIIKHTN PCGTALGSSI SEAYKKAFNA 

       310        320        330        340        350        360 
DSTSAFGGIV ALNRPIDAAT ASELTKTFLE CVVAPSCEAE AQEILAKKSN VRVLTLADLS 

       370        380        390        400        410        420 
SGPKDTVKAI AGGFLVQTAD DIVADTSQWQ VVTERQPTDS ELAELLFAWK VCKHVKSNAI 

       430        440        450        460        470        480 
VVTSDRTTLG VGAGQMNRVG SVKIALEQAG EKAKGAILAS DGFFPFDDSV RTAAAAGITA 

       490        500        510 
IVQPGGSLRD KDSIKAANDL GLLMVLTGVR HFLH 

« Hide

References

[1]"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. expand/collapse author list , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001037 Genomic DNA. Translation: ACC84589.1.
RefSeqYP_001869532.1. NC_010628.1.

3D structure databases

ProteinModelPortalB2IX54.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING63737.Npun_R6316.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACC84589; ACC84589; Npun_R6316.
GeneID6255660.
KEGGnpu:Npun_R6316.
PATRIC22766575. VBINosPun48114_7409.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0138.
HOGENOMHOG000230373.
KOK00602.
OMALKSTLRY.
OrthoDBEOG6QCDFF.
PhylomeDBB2IX54.

Enzyme and pathway databases

BioCycNPUN63737:GJNP-6182-MONOMER.
UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3D3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPMF_00139. PurH.
InterProIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR9_NOSP7
AccessionPrimary (citable) accession number: B2IX54
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: July 9, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways