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B2IWJ8

- RTCA_NOSP7

UniProt

B2IWJ8 - RTCA_NOSP7

Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 42 (01 Oct 2014)
      Sequence version 1 (10 Jun 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

    Catalytic activityi

    ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei107 – 1071ATPUniRule annotation
    Active sitei316 – 3161Tele-AMP-histidine intermediateUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi290 – 2945ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. RNA-3'-phosphate cyclase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciNPUN63737:GJNP-2855-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
    Short name:
    RNA cyclaseUniRule annotation
    Short name:
    RNA-3'-phosphate cyclaseUniRule annotation
    Gene namesi
    Name:rtcAUniRule annotation
    Ordered Locus Names:Npun_F2920
    OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
    Taxonomic identifieri63737 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
    ProteomesiUP000001191: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 348348RNA 3'-terminal phosphate cyclasePRO_1000099350Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi63737.Npun_F2920.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0430.
    HOGENOMiHOG000015264.
    KOiK01974.
    OMAiQNEGPGN.
    OrthoDBiEOG6RNQDX.
    PhylomeDBiB2IWJ8.

    Family and domain databases

    Gene3Di3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPiMF_00200. RTC.
    InterProiIPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view]
    PANTHERiPTHR11096. PTHR11096. 1 hit.
    PfamiPF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view]
    SUPFAMiSSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 1 hit.
    TIGRFAMsiTIGR03399. RNA_3prim_cycl. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    B2IWJ8-1 [UniParc]FASTAAdd to Basket

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    MIEIDGSYGE GGGQVLRTSL SLAAITGEPI RIAGIRAGRK KPGLAAQHLT    50
    AVRAAGRICN AQLRGDALGS MLLEFIPGSA VQAGIYTFDV SKAQEGGSAG 100
    AIALVLQTIL LPLALATGNS QVTLKGGTHV NFSPTVTYIE QVYLPILQRM 150
    GVEAQVKLGA WGWFPQGGGE IELQVIGGTQ LGGINLLERG ELQQVRGIAA 200
    VTELPSHIPQ RMANRAENLL REAHLKVRVQ TLREKGVAPG AGIFLTAEYQ 250
    NSLTGFGGFG RLRLSAETVA EIACQQLLEF HYTGAAVDEH LADQLLLPAT 300
    LASQESQYQV AEVSRHLITN AAVIEQFGLA QIRVNEADKI VSVKSLTS 348
    Length:348
    Mass (Da):36,859
    Last modified:June 10, 2008 - v1
    Checksum:i96962FB1C535034B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001037 Genomic DNA. Translation: ACC81452.1.
    RefSeqiYP_001866395.1. NC_010628.1.

    Genome annotation databases

    EnsemblBacteriaiACC81452; ACC81452; Npun_F2920.
    GeneIDi6252331.
    KEGGinpu:Npun_F2920.
    PATRICi22758510. VBINosPun48114_3420.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001037 Genomic DNA. Translation: ACC81452.1 .
    RefSeqi YP_001866395.1. NC_010628.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 63737.Npun_F2920.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACC81452 ; ACC81452 ; Npun_F2920 .
    GeneIDi 6252331.
    KEGGi npu:Npun_F2920.
    PATRICi 22758510. VBINosPun48114_3420.

    Phylogenomic databases

    eggNOGi COG0430.
    HOGENOMi HOG000015264.
    KOi K01974.
    OMAi QNEGPGN.
    OrthoDBi EOG6RNQDX.
    PhylomeDBi B2IWJ8.

    Enzyme and pathway databases

    BioCyci NPUN63737:GJNP-2855-MONOMER.

    Family and domain databases

    Gene3Di 3.30.360.20. 1 hit.
    3.65.10.20. 2 hits.
    HAMAPi MF_00200. RTC.
    InterProi IPR013791. RNA3'-term_phos_cycl_insert.
    IPR023797. RNA3'_phos_cyclase_dom.
    IPR000228. RNA3'_term_phos_cyc.
    IPR017770. RNA3'_term_phos_cyc_type_1.
    IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
    [Graphical view ]
    PANTHERi PTHR11096. PTHR11096. 1 hit.
    Pfami PF01137. RTC. 1 hit.
    PF05189. RTC_insert. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52913. SSF52913. 1 hit.
    SSF55205. SSF55205. 1 hit.
    TIGRFAMsi TIGR03399. RNA_3prim_cycl. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29133 / PCC 73102.

    Entry informationi

    Entry nameiRTCA_NOSP7
    AccessioniPrimary (citable) accession number: B2IWJ8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: June 10, 2008
    Last modified: October 1, 2014
    This is version 42 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3