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Protein

RNA 3'-terminal phosphate cyclase

Gene

rtcA

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.UniRule annotation

Catalytic activityi

ATP + (RNA)-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + (RNA)-3'-(2',3'-cyclophospho)-ribonucleoside.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107ATPUniRule annotation1
Active sitei316Tele-AMP-histidine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi290 – 294ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNPUN63737:G1GBR-3005-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
RNA 3'-terminal phosphate cyclaseUniRule annotation (EC:6.5.1.4UniRule annotation)
Short name:
RNA cyclaseUniRule annotation
Short name:
RNA-3'-phosphate cyclaseUniRule annotation
Gene namesi
Name:rtcAUniRule annotation
Ordered Locus Names:Npun_F2920
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000001191 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000993501 – 348RNA 3'-terminal phosphate cyclaseAdd BLAST348

Interactioni

Protein-protein interaction databases

STRINGi63737.Npun_F2920

Structurei

3D structure databases

SMRiB2IWJ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CKX Bacteria
COG0430 LUCA
HOGENOMiHOG000015264
KOiK01974
OMAiANRPKPG
OrthoDBiPOG091H0UB7
PhylomeDBiB2IWJ8

Family and domain databases

Gene3Di3.30.360.20, 1 hit
3.65.10.20, 2 hits
HAMAPiMF_00200 RTC, 1 hit
InterProiView protein in InterPro
IPR013791 RNA3'-term_phos_cycl_insert
IPR023797 RNA3'_phos_cyclase_dom
IPR037136 RNA3'_phos_cyclase_dom_sf
IPR000228 RNA3'_term_phos_cyc
IPR017770 RNA3'_term_phos_cyc_type_1
IPR013792 RNA3'P_cycl/enolpyr_Trfase_a/b
IPR036553 RPTC_insert
PANTHERiPTHR11096 PTHR11096, 1 hit
PTHR11096:SF0 PTHR11096:SF0, 1 hit
PfamiView protein in Pfam
PF01137 RTC, 1 hit
PF05189 RTC_insert, 1 hit
PIRSFiPIRSF005378 RNA3'_term_phos_cycl_euk, 1 hit
SUPFAMiSSF52913 SSF52913, 1 hit
SSF55205 SSF55205, 1 hit
TIGRFAMsiTIGR03399 RNA_3prim_cycl, 1 hit

Sequencei

Sequence statusi: Complete.

B2IWJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEIDGSYGE GGGQVLRTSL SLAAITGEPI RIAGIRAGRK KPGLAAQHLT
60 70 80 90 100
AVRAAGRICN AQLRGDALGS MLLEFIPGSA VQAGIYTFDV SKAQEGGSAG
110 120 130 140 150
AIALVLQTIL LPLALATGNS QVTLKGGTHV NFSPTVTYIE QVYLPILQRM
160 170 180 190 200
GVEAQVKLGA WGWFPQGGGE IELQVIGGTQ LGGINLLERG ELQQVRGIAA
210 220 230 240 250
VTELPSHIPQ RMANRAENLL REAHLKVRVQ TLREKGVAPG AGIFLTAEYQ
260 270 280 290 300
NSLTGFGGFG RLRLSAETVA EIACQQLLEF HYTGAAVDEH LADQLLLPAT
310 320 330 340
LASQESQYQV AEVSRHLITN AAVIEQFGLA QIRVNEADKI VSVKSLTS
Length:348
Mass (Da):36,859
Last modified:June 10, 2008 - v1
Checksum:i96962FB1C535034B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA Translation: ACC81452.1
RefSeqiWP_012409443.1, NC_010628.1

Genome annotation databases

EnsemblBacteriaiACC81452; ACC81452; Npun_F2920
KEGGinpu:Npun_F2920

Similar proteinsi

Entry informationi

Entry nameiRTCA_NOSP7
AccessioniPrimary (citable) accession number: B2IWJ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: March 28, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health