Skip Header

Contribute Send feedback
Read comments (?) or add your own

B2IVH7 (GLO2_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6
Alternative name(s):
Glyoxalase II
Short name=Glx II
Gene names
Name:gloB
Ordered Locus Names:Npun_F2829
OrganismNostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]
Taxonomic identifier63737 [NCBI]
Taxonomic lineageBacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length257 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374

Catalytic activity

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374

Cofactor

Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374

Pathway

Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374

Subunit structure

Monomer By similarity. HAMAP MF_01374

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionhydroxyacylglutathione hydrolase activity

Inferred from electronic annotation. Source: EC

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 257257Hydroxyacylglutathione hydrolase HAMAP MF_01374
PRO_1000144776

Sites

Metal binding541Zinc 1 By similarity
Metal binding561Zinc 1 By similarity
Metal binding581Zinc 2 By similarity
Metal binding591Zinc 2 By similarity
Metal binding1131Zinc 1 By similarity
Metal binding1371Zinc 1 By similarity
Metal binding1371Zinc 2 By similarity
Metal binding1751Zinc 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
B2IVH7 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: C42B006FF2B7613B

FASTA25728,966
        10         20         30         40         50         60 
MQVIRLEALS DNYIFLLYDD KRNIAAVVDP AEAQPVLKKL AELKADLVAI FNTHHHNDHV 

        70         80         90        100        110        120 
GGNKQLIEKF PQLIVYGGAE DRGRIPGQKV FLQQGDRVEF ADRIAEVIFV PGHTRAHIAY 

       130        140        150        160        170        180 
YFPPQEASET GDLFCGDTLF SGGCGRLFEG TPTQMVDSLS KLRSLPDNTR IWCAHEYTLK 

       190        200        210        220        230        240 
NLQFALTVDG DNADLQRRFN EVKAYRSRGE ATIPSLLGVE KHTNPFLRWD QASLQSTVKS 

       250 
SDGVQTFARI REMKNNF

« Hide

References

[1]"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. expand/collapse author list , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R.
Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001037 Genomic DNA. Translation: ACC81362.1.
RefSeqYP_001866305.1. NC_010628.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGB2IVH7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6252240.
GenomeReviewsGene locus Npun_F2829 in contig CP001037_GR.
KEGGnpu:Npun_F2829.
PATRIC22758288. VBINosPun48114_3309.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG753931.
OMAIGCGRVF.
PhylomeDBB2IVH7.
ProtClustDBCLSK892602.

Family and domain databases

HAMAPMF_01374. Glyoxalase_2.
[Tree]
InterProIPR001279. Beta-lactamas-like.
IPR017782. Hydroxyacylglutathione_Hdrlase.
[Graphical view]
KOK01069.
PANTHERPTHR11935:SF7. PTHR11935:SF7. 1 hit.
PfamPF00753. Lactamase_B. 1 hit.
[Graphical view]
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
TIGRFAMsTIGR03413. GSH_gloB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLO2_NOSP7
AccessionPrimary (citable) accession number: B2IVH7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents