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Protein

dITP/XTP pyrophosphatase

Gene

Npun_R4467

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotationSAAS annotation

Catalytic activityi

ITP + H2O = IMP + diphosphate.UniRule annotationSAAS annotation
XTP + H2O = XMP + diphosphate.UniRule annotationSAAS annotation
dITP + H2O = dIMP + diphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39MagnesiumUniRule annotation1
Active sitei68Proton acceptorUniRule annotation1
Metal bindingi68MagnesiumUniRule annotation1
Binding sitei69Substrate; via amide nitrogenUniRule annotation1
Binding sitei170SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotationSAAS annotationImported
Biological processNucleotide metabolismUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-bindingUniRule annotationSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:Npun_R4467Imported
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)Imported
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
Proteomesi
  • UP000001191 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi63737.Npun_R4467.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 14Substrate bindingUniRule annotation6
Regioni147 – 150Substrate bindingUniRule annotation4
Regioni175 – 176Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
KOiK02428.
OMAiYDPIFQP.
OrthoDBiPOG091H02BP.
PhylomeDBiB2IUQ9.

Family and domain databases

CDDicd00515. HAM1. 1 hit.
Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase. 1 hit.
InterProiView protein in InterPro
IPR020922. dITP/XTP_pyrophosphatase.
IPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiView protein in Pfam
PF01725. Ham1p_like. 1 hit.
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

B2IUQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKLLVVATG NPGKLREMQA YLKNSGWELT LKPEELDIEE TGETFAANAC
60 70 80 90 100
LKASQIAKAT GNWAIADDSG LQVDALNGAP GVYSARYAKT DSERIARLLK
110 120 130 140 150
ELGNEVNRQA QFVCAVAIAR PDGAIVLESE GICRGEILHA PRGDSGFGYD
160 170 180 190
PIFYVQELQL TFAEMTRELK GSISHRGKAF TALLPQLERI KRRD
Length:194
Mass (Da):21,225
Last modified:June 10, 2008 - v1
Checksum:iEDA708B3CBC12B0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC82834.1.
RefSeqiWP_012410795.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC82834; ACC82834; Npun_R4467.
KEGGinpu:Npun_R4467.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiB2IUQ9_NOSP7
AccessioniPrimary (citable) accession number: B2IUQ9
Entry historyiIntegrated into UniProtKB/TrEMBL: June 10, 2008
Last sequence update: June 10, 2008
Last modified: June 7, 2017
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported