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Protein

Non-canonical purine NTP pyrophosphatase

Gene

Npun_R4467

Organism
Nostoc punctiforme (strain ATCC 29133 / PCC 73102)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+SAAS annotation, Mn2+SAAS annotationNote: Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn2+.SAAS annotation
  • Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn2+.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi39 – 391Magnesium or manganeseUniRule annotation
Metal bindingi68 – 681Magnesium or manganeseUniRule annotation
Binding sitei150 – 1501SubstrateUniRule annotation
Binding sitei170 – 1701SubstrateUniRule annotation
Binding sitei176 – 1761SubstrateUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nucleoside-triphosphatase activity Source: InterPro
  3. nucleoside-triphosphate diphosphatase activity Source: UniProtKB-HAMAP
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. nucleoside triphosphate catabolic process Source: InterPro
  2. purine nucleotide metabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotation

Keywords - Biological processi

Nucleotide metabolismUniRule annotationSAAS annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-bindingUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciNPUN63737:GJNP-4341-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Non-canonical purine NTP pyrophosphataseUniRule annotationSAAS annotation (EC:3.6.1.19UniRule annotationSAAS annotation)
Alternative name(s):
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Gene namesi
Ordered Locus Names:Npun_R4467Imported
OrganismiNostoc punctiforme (strain ATCC 29133 / PCC 73102)Imported
Taxonomic identifieri63737 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaNostocalesNostocaceaeNostoc
ProteomesiUP000001191 Componenti: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi63737.Npun_R4467.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni9 – 146Substrate bindingUniRule annotation
Regioni68 – 692Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0127.
HOGENOMiHOG000293319.
KOiK02428.
OMAiTHNPGKV.
OrthoDBiEOG6CZQQP.
PhylomeDBiB2IUQ9.

Family and domain databases

Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

B2IUQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKLLVVATG NPGKLREMQA YLKNSGWELT LKPEELDIEE TGETFAANAC
60 70 80 90 100
LKASQIAKAT GNWAIADDSG LQVDALNGAP GVYSARYAKT DSERIARLLK
110 120 130 140 150
ELGNEVNRQA QFVCAVAIAR PDGAIVLESE GICRGEILHA PRGDSGFGYD
160 170 180 190
PIFYVQELQL TFAEMTRELK GSISHRGKAF TALLPQLERI KRRD
Length:194
Mass (Da):21,225
Last modified:June 9, 2008 - v1
Checksum:iEDA708B3CBC12B0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC82834.1.
RefSeqiYP_001867777.1. NC_010628.1.

Genome annotation databases

EnsemblBacteriaiACC82834; ACC82834; Npun_R4467.
KEGGinpu:Npun_R4467.
PATRICi22762171. VBINosPun48114_5237.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001037 Genomic DNA. Translation: ACC82834.1.
RefSeqiYP_001867777.1. NC_010628.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi63737.Npun_R4467.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC82834; ACC82834; Npun_R4467.
KEGGinpu:Npun_R4467.
PATRICi22762171. VBINosPun48114_5237.

Phylogenomic databases

eggNOGiCOG0127.
HOGENOMiHOG000293319.
KOiK02428.
OMAiTHNPGKV.
OrthoDBiEOG6CZQQP.
PhylomeDBiB2IUQ9.

Enzyme and pathway databases

BioCyciNPUN63737:GJNP-4341-MONOMER.

Family and domain databases

Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 29133 / PCC 73102Imported.

Entry informationi

Entry nameiB2IUQ9_NOSP7
AccessioniPrimary (citable) accession number: B2IUQ9
Entry historyi
Integrated into UniProtKB/TrEMBL: June 9, 2008
Last sequence update: June 9, 2008
Last modified: March 31, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.