B2IUM5 (LPXD_NOSP7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	Npun_F4432

Organism

Nostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]

Taxonomic identifier

63737 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000127689
Sites
	Active site	248	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2IUM5 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 99F6E6B62C81BDAE	FASTA	350	37,066
	10 20 30 40 50 60 MKFSEIISQF GETGTNHSLT SNPDHDPEII GVAAIDEATN GTLSYVEGPK FGSFVSKTNA 70 80 90 100 110 120 TALILPQDEK LQELAQENGI VWISTSDPRL LFAKAIALFY QPYRPVPEIH PTAVIHSSAK 130 140 150 160 170 180 VGSDVYVGPH VVIQQGVEIG DGAIIHPNVV IYPDTKIGDR TTLHANCTIH ERTRIGADCV 190 200 210 220 230 240 IHSGAVIGAE GFGFVPSRTG WLKMEQSGYT VLEDGVVVGC NTAIDRPAVG ETRVGRNTVI 250 260 270 280 290 300 DNLVQIGHGC QIGSGCAIAG QAGMAGGVKL GNRVILAGQT GIANQVKIGD GAIASAQTGI 310 320 330 340 350 HSDVAPGEIV SGTPAIPYKL YLKVCAVYSR LPDMYQSLKQ LQRKFKNSND

References

[1]

"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001037 Genomic DNA. Translation: ACC82800.1.
RefSeq	YP_001867743.1. NC_010628.1.
3D structure databases
ProteinModelPortal	B2IUM5.
ModBase	Search...
Protein-protein interaction databases
STRING	B2IUM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6253777.
GenomeReviews	Gene locus Npun_F4432 in contig CP001037_GR.
KEGG	npu:Npun_F4432.
PATRIC	22762075. VBINosPun48114_5194.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG469615.
OMA	AKISTLF.
PhylomeDB	B2IUM5.
ProtClustDB	PRK00892.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 5 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_NOSP7

Accession

Primary (citable) accession number: B2IUM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program