B2IU40 (B2IU40_NOSP7) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase HAMAP MF_00534
EC=6.1.1.22 HAMAP MF_00534

Alternative name(s):
Asparaginyl-tRNA synthetase HAMAP MF_00534

Gene names

Name:	asnS HAMAP MF_00534
Ordered Locus Names:	Npun_R0874

Organism

Nostoc punctiforme (strain ATCC 29133 / PCC 73102) [Complete proteome] [HAMAP]

Taxonomic identifier

63737 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm By similarity HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. HAMAP MF_00534

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00534
Cellular component	Cytoplasm HAMAP MF_00534
Ligand	ATP-binding HAMAP MF_00534 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00534 EMBL ACC79611.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	B2IU40 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 4D3715F6968E92B3	FASTA	463	52,830
	10 20 30 40 50 60 MVNRRIAEIL RSGQPDESFQ VQGWVRTKRE SKGFAFIEVN DGSSLANLQV VINQDLPDYE 70 80 90 100 110 120 AILKKLNTGA AVEATGVLVA SLGKGQRIEL KAESVKVYGE ADPDTYPLQK KRHSFEFLRT 130 140 150 160 170 180 IGHLRSRTNS FGAVFRVRNA CSTAIHQFFQ QRGFLWVHTP IITASDCEGA GELFSVTSLD 190 200 210 220 230 240 LKNIPRTENQ AVDYSQDFFA KPTYLTVSGQ LEAEVMAMAF SNVYTFGPTF RAENSNTSRH 250 260 270 280 290 300 LAEFWMVEPE MAFCDLEGDM DLAEAFLKHI FKYVLETCPE DMEFFNERID KSVLATAENI 310 320 330 340 350 360 INNQFERLTY TEAIKVLEKA DVKFEYPVSW GLDLQSEHER YLAEQQFKKP VIVTDYPAQI 370 380 390 400 410 420 KAFYMRLNDD EKTVRAMDIL APKIGEIVGG SQREERLEVL ERRVLAQGLK PEELWWYLDL 430 440 450 460 RRYGTVPHAG FGLGFERLVQ FMTGMGNIRD VIPFPRTPQS AEF

References

[1]

"Complete sequence of chromosome of Nostoc punctiforme ATCC 29133." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Meeks J.C. , Elhai J., Campbell E.L., Thiel T., Longmire J., Potts M., Atlas R. Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 29133 / PCC 73102.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001037 Genomic DNA. Translation: ACC79611.1.
RefSeq	YP_001864554.1. NC_010628.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2IU40.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6250329.
GenomeReviews	Gene locus Npun_R0874 in contig CP001037_GR.
KEGG	npu:Npun_R0874.
PATRIC	22753605. VBINosPun48114_1011.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG745843.
OMA	AIHRFFH.
PhylomeDB	B2IU40.
ProtClustDB	PRK03932.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B2IU40_NOSP7

Accession

Primary (citable) accession number: B2IU40

Entry history

Integrated into UniProtKB/TrEMBL:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)