B2IQ63 (ISPD_STRPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	SPCG_1235

Organism

Streptococcus pneumoniae (strain CGSP14) [Complete proteome] [HAMAP]

Taxonomic identifier

516950 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_1000094355
Sites
	Site	14	1	Transition state stabilizer By similarity
	Site	22	1	Transition state stabilizer By similarity
	Site	161	1	Positions MEP for the nucleophilic attack By similarity
	Site	218	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2IQ63 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: FA37808254BF6B21	FASTA	235	26,329
	10 20 30 40 50 60 MIYAGILAGG TGTRMGISNL PKQFLELGDR PILIHTIEKF VLEPSIEKIV VGVHGDWVSH 70 80 90 100 110 120 AEDLVDKYLP LYKERIIITK GGADRNTSIK KIIEAIDAYR PLTPEDIVVT HDSVRPFITL 130 140 150 160 170 180 RMIQDNIQLA QNHDAVDTVV EAVDTIVEST NGQFITDIPN RAHLYQGQTP QTFRCKDFMD 190 200 210 220 230 LYGSLSDEEK EIWTDACKIF VIKGKDVALA KGEYSNLKIT TVTDLKIAKS MIEKD

References

[1]

"Genome evolution driven by host adaptations results in a more virulent and antimicrobial-resistant Streptococcus pneumoniae serotype 14." Ding F., Tang P., Hsu M.-H., Cui P., Hu S., Yu J., Chiu C.-H. BMC Genomics 10:158-158(2009) [PubMed: 19361343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CGSP14.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001033 Genomic DNA. Translation: ACB90487.1.
RefSeq	YP_001835952.1. NC_010582.1.
3D structure databases
ProteinModelPortal	B2IQ63.
ModBase	Search...
Protein-protein interaction databases
STRING	B2IQ63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000017795; EBSTRP00000017109; EBSTRG00000017795.
GeneID	6218432.
GenomeReviews	Gene locus SPCG_1235 in contig CP001033_GR.
KEGG	spw:SPCG_1235.
PATRIC	19678738. VBIStrPne123335_1325.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027822.
HOGENOM	HBG672839.
OMA	GELFNIK.
ProtClustDB	PRK00155.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_STRPS

Accession

Primary (citable) accession number: B2IQ63

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program