B2IKA4 (SYD_BEII9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	Bind_1396

Organism

Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) [Complete proteome] [HAMAP]

Taxonomic identifier

395963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Beijerinckia

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000090960

Sequences

	Sequence		Length	Mass (Da)
	B2IKA4 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: DC444B5FC98960BE	FASTA	597	66,892
	10 20 30 40 50 60 MHRYRTHTCG DLREAQADET TRLSGWCHRI RDHGGVLFID LRDHYGITQC VVDPDSKAFP 70 80 90 100 110 120 LAEKLRSEWV VRIDGLVRKR PSGTENPEMP TGFIEIYVTE IEVLGAAAEL PMPVFGDIDY 130 140 150 160 170 180 PEDIRLKYRF LDLRREKLHK NIMLRGQVID SLRRRMKEQG FFEFQTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRIHAGK FYALPQAPQQ YKQLLMMAGF DRYFQIAPCF RDEDPRADRL PGEFYQLDVE 250 260 270 280 290 300 MSFITQEDVF AAMEPVIRGA FEEFGNGQPV TQTFPRIPYA EAMLKYGSDK PDLRNPLVIA 310 320 330 340 350 360 DVTDLFARDD VSFNAFKNVI KKGGVVRAIP ATGAASQPRS FFDKLNDWAK SEGAAGLGYV 370 380 390 400 410 420 IFEGSDEAPV GKGPIAKFLP AEVQAAIVTR ADLKAGDAVF FACDIEEKAA KLAGAARLRI 430 440 450 460 470 480 GHELGLSKTG VFELCWIVDF PMYEWNEDDK TVDFSHNPFS MPQGGLEALQ TQDPLTIKAF 490 500 510 520 530 540 QYDIACNGYE IASGGIRNHR PEAMVKAFEI AGYGEETVVE RFGGMYRAFQ YGAPPHGGMA 550 560 570 580 590 AGVDRIIMLL AGVQNLREIS LFPMNQKAED LLMGAPSEAT TKQLRELHIR LNLPEPK

References

[1]

"Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N. , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9039 / DSM 1715 / NCIB 8712.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001016 Genomic DNA. Translation: ACB95036.1.
RefSeq	YP_001832525.1. NC_010581.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2IKA4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6199281.
GenomeReviews	Gene locus Bind_1396 in contig CP001016_GR.
KEGG	bid:Bind_1396.
PATRIC	21085607. VBIBeiInd21058_1744.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG396032.
OMA	YQLDVEM.
ProtClustDB	PRK00476.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BEII9

Accession

Primary (citable) accession number: B2IKA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program