Reviewed, UniProtKB/Swiss-Prot B2IJH6 (ILVD_BEII9)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase
      Short name=DAD
    EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Bind_2717

Organism

Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) [Complete proteome] [HAMAP]

Taxonomic identifier

395963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Beijerinckia

Protein attributes

Sequence length	617 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 617	617	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089370
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	B2IJH6-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 10C88EF29BA6EDBB	FASTA	617	65,616
	10 20 30 40 50 60 MPDYRSRTTT HGRNMAGARG LWRATGVKDE DFGKPIIAIA NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AGEIEKAGGI AKEFNTIAVD DGIAMGHDGM LYSLPSRELI ADSVEYMVNA HCADALVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPT IFVSGGPMEA GKAQVGGKTL ALDLVDAMVA AADDRVSAED 190 200 210 220 230 240 VAVIERSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS VLATHADRRR LFVEAGHLIV 250 260 270 280 290 300 DLARRYYEQN DGSVLPRAIA SFKAFENAMT LDIAMGGSTN TVLHLLAAAH EAGVPFMMAD 310 320 330 340 350 360 IDRLSRGVPC LCKVAPAKND VHMEDVHRAG GIMAILGELD RAGLLHADLP TVHSSSLGEA 370 380 390 400 410 420 LMRWDIRRSN SEMVQTFYKA APGNVPTTQA FSQEKRYEAL DLDRETGVIR DQAHAFSQDG 430 440 450 460 470 480 GLAVLFGNLA EEGSIVKTAG VDESILTFAG PARVFESQDS AVEAILAGRI VAGDVVVIRY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SKGLGKACAL ITDGRFSGGT SGLSIGHVSP EAAEGGMIAL 550 560 570 580 590 600 VEEGDRIEID IKTRSLILAV PEAELARRRG VMEAKGANAF KPRERQRQVS TALRAYAAFT 610 TSAARGAVRD LDQKPRF

References

[1]

"Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N. , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001016 Genomic DNA. Translation: ACB96289.1.
RefSeq	YP_001833778.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6199305.
GenomeReviews	Gene locus Bind_2717 in contig CP001016_GR.
KEGG	bid:Bind_2717.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	SRKVPCL.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_BEII9

Accession

Primary (citable) accession number: B2IJH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)