Reviewed, UniProtKB/Swiss-Prot B2II34 (KATG_BEII9)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Bind_0973

Organism

Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) [Complete proteome] [HAMAP]

Taxonomic identifier

395963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Beijerinckia

Protein attributes

Sequence length	738 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 738	738	Catalase-peroxidase HAMAP MF_01961		PRO_0000354725
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	268	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 227		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-253) By similarity
	Cross-link	227 ↔ 253		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2II34-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 64F66F5F552AA5FB	FASTA	738	80,352
	10 20 30 40 50 60 MDAQTDDKAQ GKCPFVHAEH GRTNRDWWPK QPNLQILHQN SSLSNPMGEA FDYAKEFKSL 70 80 90 100 110 120 DLNAVIADLK ALMTDSQDWW PADFGHYGGL FIRMAWHSAG TYRIGDGRGG AGGGQQRFAP 130 140 150 160 170 180 LNSWPDNVSL DKARRLLWPI KQKYGRKISW ADLMILTGNV ALESMGFKTF GFAGGRIDTW 190 200 210 220 230 240 EPDQSAYWGA ETTWLALSND PNNKHSRYSG DRDLENPLAA VQMGLIYVNP EGPDGNPDPL 250 260 270 280 290 300 AAARDIRETF ARMAMNDEET VALIAGGHTF GKTHGAGPAD HVGSEPEAAA VEQQGLGWKS 310 320 330 340 350 360 SFGTGVGKDA ITSGLEVIWT QTPIQWSNHY LENLFGFEWE LTKSPAGAHQ WRPKNGAGAG 370 380 390 400 410 420 SVPDAFDPNK RHAPSMLTTD LALRADPAYE KIARRYLENP AEFADAFARA WFKLTHRDMG 430 440 450 460 470 480 PRARYLGPLV PEEELIWQDP VPAVDHPLVD EADVVALKEK ILSSGLTVAE LVSTAWASAS 490 500 510 520 530 540 TFRGSDKRGG ANGARILLAP QKDWAVNEPQ KLDKVLKALE VIQADFNAAL SGGKKISLAD 550 560 570 580 590 600 LIILAGGAAI EKAASEAGQK ITVPFAPGRT DASQEQTDVH SFAPLEPPAD GFRNDVPAKL 610 620 630 640 650 660 AEISGHLLVD RAQLLTLTAP EMTVLIGGLR VLGANTGGSK HGVFTTRPGV LSNDFFTNLL 670 680 690 700 710 720 DMGTEWKPAA EDGVYEGHDR KTKAVKWTAT RVDLLFGSHA QLRALAEVYG CADAQTKFVT 730 DFVAAWTKVM NADRFDLI

References

[1]

"Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N. , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001016 Genomic DNA. Translation: ACB94617.1.
RefSeq	YP_001832106.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6200656.
GenomeReviews	Gene locus Bind_0973 in contig CP001016_GR.
KEGG	bid:Bind_0973.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FEWELTK.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_BEII9

Accession

Primary (citable) accession number: B2II34

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)