B2IHX3 (SYA_BEII9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Bind_2405

Organism

Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) [Complete proteome] [HAMAP]

Taxonomic identifier

395963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Beijerinckia

Protein attributes

Sequence length	898 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence ACB96016.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 898	898	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347506
Sites
	Metal binding	564	1	Zinc Potential
	Metal binding	568	1	Zinc Potential
	Metal binding	682	1	Zinc Potential
	Metal binding	686	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	B2IHX3 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 5121E2BAF059BC1C	FASTA	898	97,547
	10 20 30 40 50 60 MNGVNEIRAA FLDFFRKNGH EIVPSSPLVP RNDPTLMFTN AGMVQFKNLF TGVEKRPYTR 70 80 90 100 110 120 ASTAQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKPLAIELA WKLITEVFDL 130 140 150 160 170 180 PKDRLLVTVY HDDDEAYDLW KKIAGFPDSK IIRIATSDNF WAMGDTGPCG PCSEIFYDQG 190 200 210 220 230 240 EKLFGGPPGS ADEDGDRFLE FWNLVFMQFE QRGPGDRIAL PKPSIDTGMG LERIAALLQG 250 260 270 280 290 300 VTSNYDIDLM RALILAVAQA TGVDPDGPMR ASHRVIADHL RASSFLIADG VLPSNEGRGY 310 320 330 340 350 360 VLRRIMRRAM RHAQLLGAQE PLLWRLVPAL TREMGQAYPE LLRAEALIVE TLRLEETRFR 370 380 390 400 410 420 ATLARGLTIL EDETRHLSEG GVLSGEVAFK LYDTYGFPLD LTQDALRAKS LGVDMEGFEK 430 440 450 460 470 480 AMARQRAEAR KAWSGSGEAA TDTHWFALRE QLGATDFLGY DTEKAEALIQ AILQDGKEVL 490 500 510 520 530 540 RLEAGQSGAI VLNQTPFYGE SGGQVGDTGL IIAPGMRFKV ENTHKKLGDL FIHEGIVEEG 550 560 570 580 590 600 AVVPGLEVRT LVDHSRRTAV RANHSATHLL HEALRQVLGD HIAQKGSLVA PDRLRFDFSH 610 620 630 640 650 660 PKPVTPEEWT QIEDIANSVI LENAPVTTKL MSIEDAMGSG ARALFGEKYG DEVRVVSMGY 670 680 690 700 710 720 DEDKADDSTD GRIAPPFSVE LCGGTHVART GDIGLLTIIS ESAVAAGVRR IEAKTGSSAR 730 740 750 760 770 780 HHLNTQASLL HSLAAQLKSP EDEASKRLSA LIEERRKLDR ELSEARKKLA MGGGSSNGAD 790 800 810 820 830 840 SPLREIGGIK FFRRAVTGVE MKDLKSLADE AKQTIGSGIV AIVGVGSDNK ASVVVGVTDD 850 860 870 880 890 LIDRFDAVAL VRLAAGKLGG KGGGGRKDLA QAGGPDGEAA EAALTAIEES LGSSLPTP

References

[1]

"Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N. , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9039 / DSM 1715 / NCIB 8712.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001016 Genomic DNA. Translation: ACB96016.1. Different initiation.
RefSeq	YP_001833505.1. NC_010581.1.
3D structure databases
ProteinModelPortal	B2IHX3.
ModBase	Search...
Protein-protein interaction databases
STRING	B2IHX3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6199545.
GenomeReviews	Gene locus Bind_2405 in contig CP001016_GR.
KEGG	bid:Bind_2405.
PATRIC	21087739. VBIBeiInd21058_2793.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG354397.
ProtClustDB	PRK00252.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BEII9

Accession

Primary (citable) accession number: B2IHX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program