Reviewed, UniProtKB/Swiss-Prot B2IHX3 (SYA_BEII9)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	Bind_2405

Organism

Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) [Complete proteome] [HAMAP]

Taxonomic identifier

395963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Beijerinckia

Protein attributes

Sequence length	898 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 898	898	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347506

Sequences

	Sequence		Length	Mass (Da)
	B2IHX3-1 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: 5121E2BAF059BC1C	FASTA	898	97,547
	10 20 30 40 50 60 MNGVNEIRAA FLDFFRKNGH EIVPSSPLVP RNDPTLMFTN AGMVQFKNLF TGVEKRPYTR 70 80 90 100 110 120 ASTAQKCVRA GGKHNDLDNV GYTARHHTFF EMLGNFSFGD YFKPLAIELA WKLITEVFDL 130 140 150 160 170 180 PKDRLLVTVY HDDDEAYDLW KKIAGFPDSK IIRIATSDNF WAMGDTGPCG PCSEIFYDQG 190 200 210 220 230 240 EKLFGGPPGS ADEDGDRFLE FWNLVFMQFE QRGPGDRIAL PKPSIDTGMG LERIAALLQG 250 260 270 280 290 300 VTSNYDIDLM RALILAVAQA TGVDPDGPMR ASHRVIADHL RASSFLIADG VLPSNEGRGY 310 320 330 340 350 360 VLRRIMRRAM RHAQLLGAQE PLLWRLVPAL TREMGQAYPE LLRAEALIVE TLRLEETRFR 370 380 390 400 410 420 ATLARGLTIL EDETRHLSEG GVLSGEVAFK LYDTYGFPLD LTQDALRAKS LGVDMEGFEK 430 440 450 460 470 480 AMARQRAEAR KAWSGSGEAA TDTHWFALRE QLGATDFLGY DTEKAEALIQ AILQDGKEVL 490 500 510 520 530 540 RLEAGQSGAI VLNQTPFYGE SGGQVGDTGL IIAPGMRFKV ENTHKKLGDL FIHEGIVEEG 550 560 570 580 590 600 AVVPGLEVRT LVDHSRRTAV RANHSATHLL HEALRQVLGD HIAQKGSLVA PDRLRFDFSH 610 620 630 640 650 660 PKPVTPEEWT QIEDIANSVI LENAPVTTKL MSIEDAMGSG ARALFGEKYG DEVRVVSMGY 670 680 690 700 710 720 DEDKADDSTD GRIAPPFSVE LCGGTHVART GDIGLLTIIS ESAVAAGVRR IEAKTGSSAR 730 740 750 760 770 780 HHLNTQASLL HSLAAQLKSP EDEASKRLSA LIEERRKLDR ELSEARKKLA MGGGSSNGAD 790 800 810 820 830 840 SPLREIGGIK FFRRAVTGVE MKDLKSLADE AKQTIGSGIV AIVGVGSDNK ASVVVGVTDD 850 860 870 880 890 LIDRFDAVAL VRLAAGKLGG KGGGGRKDLA QAGGPDGEAA EAALTAIEES LGSSLPTP

References

[1]

"Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N. , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001016 Genomic DNA. Translation: ACB96016.1. Different initiation.
RefSeq	YP_001833505.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6199545.
GenomeReviews	Gene locus Bind_2405 in contig CP001016_GR.
KEGG	bid:Bind_2405.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00036. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_cons-reg. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BEII9

Accession

Primary (citable) accession number: B2IHX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	September 2, 2008
Last modified:	November 24, 2009
This is version 11 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)