Reviewed, UniProtKB/Swiss-Prot B2IGF7 (MRAY_BEII9)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	Bind_0689

Organism

Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) [Complete proteome] [HAMAP]

Taxonomic identifier

395963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Beijerinckia

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity. HAMAP MF_00038
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00038.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000090592
Regions
	Transmembrane	27 – 47	21	Potential
	Transmembrane	72 – 92	21	Potential
	Transmembrane	94 – 114	21	Potential
	Transmembrane	135 – 155	21	Potential
	Transmembrane	167 – 187	21	Potential
	Transmembrane	199 – 219	21	Potential
	Transmembrane	236 – 256	21	Potential
	Transmembrane	263 – 283	21	Potential
	Transmembrane	289 – 309	21	Potential
	Transmembrane	337 – 357	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	B2IGF7-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 03BDA2754CBEC807	FASTA	360	38,699
	10 20 30 40 50 60 MLTWLAEFAV YFTPLNLFRY ITFRTGGAIA TALFFVFFFG PNIIKSLRIK QGKGQPIRSD 70 80 90 100 110 120 GPQSHLLTKK GTPTMGGLMI LSGLFVSTLL WANLSNHYVW VVLWVMLGYG AIGFYDDYLK 130 140 150 160 170 180 VTKQTHNGFS GRGRLACEAG VALVACIAMM KLGTPHMTSL AFPAVNGYVV DLGLFFLIFG 190 200 210 220 230 240 PFVIVASGNA VNLTDGLDGL AIVPVMIAAG TFGIIAYLVG NAIYSDYLRI NFVPGAGELA 250 260 270 280 290 300 VVSGAVIGAG LGFLWFNAPP AQIFMGDTGS LALGGLLGTI AVAIKHEMVL AIVGGLFALE 310 320 330 340 350 360 TLSVIVQVVS FKLTGKRVFK MAPIHHHFEQ LGWSEPQVVV RFWIIAFVLA LVGLSTLKLR

References

[1]

"Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N. , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001016 Genomic DNA. Translation: ACB94339.1.
RefSeq	YP_001831828.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6201774.
GenomeReviews	Gene locus Bind_0689 in contig CP001016_GR.
KEGG	bid:Bind_0689.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG708263.
OMA	LQVFYFK.
Family and domain databases
HAMAP	MF_00038. MraY. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. PF10555. MraY_sig1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_BEII9

Accession

Primary (citable) accession number: B2IGF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)