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B2IEZ6

- BIOB_BEII9

UniProt

B2IEZ6 - BIOB_BEII9

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Protein

Biotin synthase

Gene

bioB

Organism
Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi64 – 641Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi105 – 1051Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi137 – 1371Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi197 – 1971Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi269 – 2691Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBIND395963:GJA7-538-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Bind_0535
OrganismiBeijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712)
Taxonomic identifieri395963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeBeijerinckia
ProteomesiUP000001695: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Biotin synthasePRO_0000381237Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi395963.Bind_0535.

Structurei

3D structure databases

ProteinModelPortaliB2IEZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B2IEZ6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNAPFVVSPV RHDWSRHEIK ALYDLPLLEL IARASQIHAL YHDPNDLQKA
60 70 80 90 100
SLLSIKTGGC SEDCGYCSQS ARRDEVHLDR VEMMSPSDVL AVAAQARDNG
110 120 130 140 150
ADRFCMGAAW RQVRDGAAFD AVLEMVEGVR ALGMEACVTL GMLNQSQAQR
160 170 180 190 200
LKQAGLTAYN HNLDTSPEFY PQIVTTHTYD ERLATLEAVR GEGIALCCGG
210 220 230 240 250
IIGMGETVED RVALLAILAG FDPHPESVPI NALVPVAGTP LGDRQKLDPL
260 270 280 290 300
EIVRMIATAR LVMPDSRIRL SAGRSSLSRE AQILCMVAGA NSIFSGNVLL
310 320
TTPNASLDAD EALMEALAPR
Length:320
Mass (Da):34,562
Last modified:June 10, 2008 - v1
Checksum:i8B21AFB29F524E84
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001016 Genomic DNA. Translation: ACB94187.1.
RefSeqiWP_012383545.1. NC_010581.1.
YP_001831676.1. NC_010581.1.

Genome annotation databases

EnsemblBacteriaiACB94187; ACB94187; Bind_0535.
GeneIDi6200045.
KEGGibid:Bind_0535.
PATRICi21083701. VBIBeiInd21058_0808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001016 Genomic DNA. Translation: ACB94187.1 .
RefSeqi WP_012383545.1. NC_010581.1.
YP_001831676.1. NC_010581.1.

3D structure databases

ProteinModelPortali B2IEZ6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395963.Bind_0535.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB94187 ; ACB94187 ; Bind_0535 .
GeneIDi 6200045.
KEGGi bid:Bind_0535.
PATRICi 21083701. VBIBeiInd21058_0808.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci BIND395963:GJA7-538-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N.
    , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 9039 / DSM 1715 / NCIB 8712.

Entry informationi

Entry nameiBIOB_BEII9
AccessioniPrimary (citable) accession number: B2IEZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: June 10, 2008
Last modified: November 26, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3