Reviewed, UniProtKB/Swiss-Prot B2IE09 (GLPK_BEII9)

Names and origin

Protein names

Recommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	Bind_0380

Organism

Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) [Complete proteome] [HAMAP]

Taxonomic identifier

395963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Beijerinckia

Protein attributes

Sequence length	507 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism By similarity.
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glycerol kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 507	507	Glycerol kinase HAMAP MF_00186		PRO_1000098716
Regions
	Nucleotide binding	417 – 421	5	ATP By similarity
Sites
	Binding site	12	1	Substrate By similarity
	Binding site	16	1	ATP By similarity
	Binding site	82	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Binding site	272	1	ATP By similarity
	Binding site	316	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2IE09-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 7E256793CAEE4535	FASTA	507	55,758
	10 20 30 40 50 60 MTKYVAVIDQ GTTSTRCMVF NKQGEIVAQH QLEHEQICPQ AGWVEHDPLE IWERTKDVIH 70 80 90 100 110 120 GSVAKAGLVA ADIAAIGITN QRETTMIWNR KTGQPYGNAI VWQDTRTDIV CNQMSAEGGQ 130 140 150 160 170 180 NRFQAKTGLP LATYFSGPKI RWMLDHYPGL RQDAEKGEAL FGNMDSWLIW KLTGGPGPAA 190 200 210 220 230 240 VHVTDVTNAS RTMLMNLKTL DWDEELLSAF AIPRAMLPSI RPSSDPEFYG FTRQDGPFGG 250 260 270 280 290 300 EIPICGDLGD QQAATVGQVC FDAGEAKNTY GTGCFMLMNT GTEMVHSNHG LLTTVCYQFG 310 320 330 340 350 360 HEKPHYALEG SVAIAGALVQ WLRDRMRVIN TAADIENLAK LVPDNGGMYF VPAFSGLFAP 370 380 390 400 410 420 YWRSEARGLI IGMTRFINRG HFARASLEAA AYQTREVVDA MQADSNVSLK VLKVDGGMTA 430 440 450 460 470 480 NDLLMQIQAD VLGVPVVRPK VAESTALGAA YAAGLAIGYW KNSDDLCQNW GVDKSWKPSG 490 500 DDTLRTKNYA MWKKAVTRTF DWLDESA

References

[1]

"Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N. , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP001016 Genomic DNA. Translation: ACB94033.1.
RefSeq	YP_001831522.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6199560.
GenomeReviews	Gene locus Bind_0380 in contig CP001016_GR.
KEGG	bid:Bind_0380.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LWKKAVT.
Family and domain databases
HAMAP	MF_00186. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_BEII9

Accession

Primary (citable) accession number: B2IE09

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)