Reviewed, UniProtKB/Swiss-Prot B2IDK3 (DXS_BEII9)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	Bind_1811

Organism

Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) [Complete proteome] [HAMAP]

Taxonomic identifier

395963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Beijerinckia

Protein attributes

Sequence length	642 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity. HAMAP MF_00315
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 642	642	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000115722

Sequences

	Sequence		Length	Mass (Da)
	B2IDK3-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: B700FFFB619F8169	FASTA	642	68,695
	10 20 30 40 50 60 MRTDSKTPLL DTIATPTDLR RLKESDLAQL ADELRTETID AVSVTGGHLG AGLGVVELTV 70 80 90 100 110 120 ALHYVFDTPH DRLIWDVGHQ AYPHKILTGR RDRIRTLRRA GGLSGFTKRS ESVYDPFGTA 130 140 150 160 170 180 HSSTSISAGL GMAAATTLSG GNNNVIAVIG DGSMSAGMAY EAMNNAGAMH SRLIIILNDN 190 200 210 220 230 240 EMSIAPPAGA LSAYLARLVS GGTYRTVREA AKQLGRHLPH FIYDRARKTE EFARNFWAGG 250 260 270 280 290 300 TMFEELGIYY VGPIDGHNLD HLLPVLKNVR DFKETGPILV HVVTQKGKGY APAAAASDKY 310 320 330 340 350 360 HSVNTFDVVT GVQTKPKANA PSYTRVFADA LVKEAERDDK IVAITAAMPG GTGLDVFGKA 370 380 390 400 410 420 FPERTFDVGI AEQHAVTFAA GLATEGYRPF CALYSTFLQR GYDQVVHDVA IQNLPVRFAI 430 440 450 460 470 480 DRAGLVGADG ATHAGSFDVS YLGILPNMVI MAAADEAELV HMVATAAAYD EGPIAFRYPR 490 500 510 520 530 540 GEGVGIELPQ IGEILDIGRG RIVREGTTVA LLSLGTRLAE ASKAAEQLAS YGISVTVADA 550 560 570 580 590 600 RFAKPLDLDL VRRLAKNHEV LITIEEGSVG GFGSFVMQAL AEEGLLDGTG LKFRSMVLPD 610 620 630 640 TFLDHDKPEK LYAQAGLDAK GIVAKVLATL GRENEAHQSL IA

References

[1]

"Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N. , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001016 Genomic DNA. Translation: ACB95439.1.
RefSeq	YP_001832928.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6201246.
GenomeReviews	Gene locus Bind_1811 in contig CP001016_GR.
KEGG	bid:Bind_1811.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	QRFPDRY.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BEII9

Accession

Primary (citable) accession number: B2IDK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)