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B2ICY4

- PNP_BEII9

UniProt

B2ICY4 - PNP_BEII9

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi489 – 4891MagnesiumUniRule annotation
Metal bindingi495 – 4951MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciBIND395963:GJA7-3245-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Bind_3192
OrganismiBeijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712)
Taxonomic identifieri395963 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBeijerinckiaceaeBeijerinckia
ProteomesiUP000001695: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 715715Polyribonucleotide nucleotidyltransferasePRO_0000381866Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi395963.Bind_3192.

Structurei

3D structure databases

ProteinModelPortaliB2ICY4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini556 – 61560KHUniRule annotationAdd
BLAST
Domaini625 – 69369S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiHGNFKSN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2ICY4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHMFEIHREE IEWAGRKLVL ETGKIARQAD GAIFATYGET TVLATVVSAK
60 70 80 90 100
SVKPGVDFFP LTVNYQEKAF AAGRIPGGYF KREGRPSERE TLVSRLIDRP
110 120 130 140 150
IRPLFPEGYR NETQVIVTVL AHDLENDPDI LSMVASSAAL TLSGVPFMGP
160 170 180 190 200
VGAARVAYVN GQYKINPTLD EVKDTALDLV VAGTSDAVLM VESEAKELSE
210 220 230 240 250
DVMLGAVMAG HSSFQPVIDA IIRLAEKAAK EPRDLILPDK TEVEVAVLAI
260 270 280 290 300
AENDLREAYK ITSKQDRYAA VDAVKAKVFG DLLPAEGEAK FPKDLVAEVF
310 320 330 340 350
HDLQAKVVRW NILDNGIRID GRDVKTVRPI LAEVGILPRA HGSALFTRGE
360 370 380 390 400
TQALVVATLG TGEDEQLIDN LEGTYRERFL LHYNFPPYSV GEAGRMGSPG
410 420 430 440 450
RREIGHGKLA WRAIHPMLPT PAEFPYTIRV VSEITESNGS SSMATVCGSS
460 470 480 490 500
LALMDAGVPL KRPTAGIAMG LILEGERYAV LSDILGDEDH LGDMDFKVAG
510 520 530 540 550
TEEGITSLQM DIKITGINEE IMRVALGQAK EGRLHILGEM AKAITGARAE
560 570 580 590 600
LGEFAPRIET LRIPTEKIRE VIGTGGKVIR EICEKTGAKI NIEDDGTVKV
610 620 630 640 650
ASSDGNSIKA AINWIKSIAT DPEVGHIYDG TVVKVVDFGA FVNFFGSKDG
660 670 680 690 700
LVHISQLAKG RVAKTSDVVK EGDKVKVKLL GFDDRGKVRL SMRLVDQETG
710
EDLEGKEPPQ AEAGE
Length:715
Mass (Da):77,499
Last modified:June 10, 2008 - v1
Checksum:i5E5E86D4AB815143
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001016 Genomic DNA. Translation: ACB96752.1.
RefSeqiYP_001834241.1. NC_010581.1.

Genome annotation databases

EnsemblBacteriaiACB96752; ACB96752; Bind_3192.
GeneIDi6198522.
KEGGibid:Bind_3192.
PATRICi21089445. VBIBeiInd21058_3631.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001016 Genomic DNA. Translation: ACB96752.1 .
RefSeqi YP_001834241.1. NC_010581.1.

3D structure databases

ProteinModelPortali B2ICY4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395963.Bind_3192.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB96752 ; ACB96752 ; Bind_3192 .
GeneIDi 6198522.
KEGGi bid:Bind_3192.
PATRICi 21089445. VBIBeiInd21058_3631.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
KOi K00962.
OMAi HGNFKSN.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci BIND395963:GJA7-3245-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N.
    , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 9039 / DSM 1715 / NCIB 8712.

Entry informationi

Entry nameiPNP_BEII9
AccessioniPrimary (citable) accession number: B2ICY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: June 10, 2008
Last modified: November 26, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3