B2ICM9 (PURA_BEII9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylosuccinate synthetase
Short name=AMPSase
Short name=AdSS
EC=6.3.4.4

Alternative name(s):
IMP--aspartate ligase

Gene names

Name:	purA
Ordered Locus Names:	Bind_0262

Organism

Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) [Complete proteome] [HAMAP]

Taxonomic identifier

395963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Beijerinckia

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity. HAMAP MF_00011
Subcellular location	Cytoplasm By similarity HAMAP MF_00011.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 431	431	Adenylosuccinate synthetase HAMAP MF_00011		PRO_1000089269
Regions
	Nucleotide binding	12 – 18	7	GTP By similarity
	Nucleotide binding	40 – 42	3	GTP By similarity
	Nucleotide binding	332 – 334	3	GTP By similarity
	Nucleotide binding	414 – 416	3	GTP By similarity
	Region	13 – 16	4	IMP binding By similarity
	Region	38 – 41	4	IMP binding By similarity
	Region	300 – 306	7	Substrate binding By similarity
Sites
	Active site	13	1	Proton acceptor By similarity
	Active site	41	1	Proton donor By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity
	Binding site	131	1	IMP By similarity
	Binding site	145	1	IMP; shared with dimeric partner By similarity
	Binding site	225	1	IMP By similarity
	Binding site	240	1	IMP By similarity
	Binding site	304	1	IMP By similarity
	Binding site	306	1	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2ICM9 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 44A913822C63E03F	FASTA	431	46,542
	10 20 30 40 50 60 MANVVVVGAQ WGDEGKGKIV DWLSIEADIV VRFQGGHNAG HTLVIGNQVY KLALLPSGIV 70 80 90 100 110 120 RLGKLSVIGN GVVVDPQHLV EEIAKLAAQG IEITPDNLKI AENVPLILGL HRELDAHRES 130 140 150 160 170 180 STVEGVKIGT TKRGIGPAYE DKVGRRAIRL MDLAEPDTLD EKIVRLLAHH EPLRRGLGLE 190 200 210 220 230 240 PISAGAIRAE LESLAPKILP FMDATWDLLE NARRAGKRIL FEGAQGALLD IDHGTYPFVT 250 260 270 280 290 300 SSNTVAANAA TGSGLGPKAI GYVLGIAKAY TTRVGGGPFP TELLDETGQL IGDRGHEYGV 310 320 330 340 350 360 NTGRRRRCGW FDAVLVRQTV KTCGIDGIAL TKLDILDGFK EIKVCVGYDL DGRRIDRLPA 370 380 390 400 410 420 SQSAQARVKP IYETIDGWEG TTAGARSWAD LPAQAIKYVR RIEELIEAPV ALLSTSPERA 430 DTILVHDPFR D

References

[1]

"Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N. , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9039 / DSM 1715 / NCIB 8712.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001016 Genomic DNA. Translation: ACB93918.1.
RefSeq	YP_001831407.1. NC_010581.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B2ICM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6199228.
GenomeReviews	Gene locus Bind_0262 in contig CP001016_GR.
KEGG	bid:Bind_0262.
PATRIC	21083095. VBIBeiInd21058_0506.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG658237.
OMA	YDELMQV.
ProtClustDB	CLSK2613283.
Family and domain databases
HAMAP	MF_00011. Adenylosucc_synth. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
KO	K01939.
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. PurA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_BEII9

Accession

Primary (citable) accession number: B2ICM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program