B2IB12 (ACSA_BEII9) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase
EC=6.2.1.1

Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme

Gene names

Name:	acsA
Ordered Locus Names:	Bind_0053

Organism

Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) [Complete proteome] [HAMAP]

Taxonomic identifier

395963 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Beijerinckiaceae › Beijerinckia

Protein attributes

Sequence length	645 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 645	645	Acetyl-coenzyme A synthetase HAMAP MF_01123		PRO_1000137259
Sites
	Active site	515	1	By similarity
Amino acid modifications
	Modified residue	607	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2IB12 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 207BBEF735EE4221	FASTA	645	70,885
	10 20 30 40 50 60 MSDKTYPVSA YWNERAYINA AKYKDMYKRS IEEPDLFWGE EGKRIDWIKP YTKVKNTSFD 70 80 90 100 110 120 PANVDIRWFE DGITNVAYNC IDRHLATRGD QTAILFEGDD PADSRAITYK ELHDSVCRLA 130 140 150 160 170 180 NVLKAHGVGK GDTVSLYLPM IPEAAFAMLA CARIGAIHSV IFGGFSPDSL AGRIEGCRSK 190 200 210 220 230 240 VLITADEGLR GGRKVPLKAN ADLAIAKTGD IVQTMIVVTR TGGAVDWVEG RDVRYEEAIA 250 260 270 280 290 300 AASPECPLTE VEAEHPLFIL YTSGSTGAPK GVVHCTGGYL VYASMTHQYV FDYHDGDVYW 310 320 330 340 350 360 CTADVGWVTG HSYIVYGPLA NGATTLMFEG VPNYPSVSRF WEVIDKHKVT IFYTAPTAIR 370 380 390 400 410 420 ALMGSGEAPV KKTSRASLRL LGSVGEPINP EAWEWYHRVV GEERCPIVDT WWQTETGGIL 430 440 450 460 470 480 ITPLPGATPE KPGSATLPFF GVKPQVVDAT GAVLEGVCEG NLVIADSWPG QMRTIFGDHD 490 500 510 520 530 540 RFVQSYFSTY PGKYFTGDGC RRDADGYYWI TGRVDDVINV SGHRLGTAEV ESSLVAHELV 550 560 570 580 590 600 SEAAVVGYPH DIKGQGIYAY VTLMNGIEPS DRLRTELVTW VRKDIGPIAT PDVVHFATGL 610 620 630 640 PKTRSGKIMR RILRKIAEKE FSALGDVSTL ADPTIVDDLI RNRLG

References

[1]

"Complete sequence of chromosome of Beijerinckia indica subsp. indica ATCC 9039." Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H., Bruce D., Goodwin L., Pitluck S., LaButti K., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Dunfield P.F., Dedysh S.N. , Liesack W., Saw J.H., Alam M., Chen Y., Murrell J.C., Richardson P. Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 9039 / DSM 1715 / NCIB 8712.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001016 Genomic DNA. Translation: ACB93712.1.
RefSeq	YP_001831201.1. NC_010581.1.
3D structure databases
ProteinModelPortal	B2IB12.
ModBase	Search...
Protein-protein interaction databases
STRING	B2IB12.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6201433.
GenomeReviews	Gene locus Bind_0053 in contig CP001016_GR.
KEGG	bid:Bind_0053.
PATRIC	21082651. VBIBeiInd21058_0284.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	TRGTEES.
ProtClustDB	PRK00174.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA_BEII9

Accession

Primary (citable) accession number: B2IB12

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program