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B2I9C6 (TRMB_XYLF2) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:XfasM23_1982
OrganismXylella fastidiosa (strain M23) [Complete proteome] [HAMAP]
Taxonomic identifier405441 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella

Protein attributes

Sequence length244 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 244244tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136375

Regions

Region223 – 2264Substrate binding By similarity

Sites

Active site1501 By similarity
Binding site751S-adenosyl-L-methionine By similarity
Binding site1001S-adenosyl-L-methionine By similarity
Binding site1271S-adenosyl-L-methionine By similarity
Binding site1501S-adenosyl-L-methionine By similarity
Binding site1541Substrate By similarity
Binding site1861Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
B2I9C6 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 8C0C67D4A753D01C

FASTA24428,322
        10         20         30         40         50         60 
MMNLLSSDGV QVLPRPFTLN ERRREVRSFV LRQGHFTPAQ KRAFDHYWPR FGVDFIGQLR 

        70         80         90        100        110        120 
DLDVLFGRSA PKVLEVGFGN GAALRFAAQH EPRYDYIGIE VYAPGVGRLL NGLAEDGSRH 

       130        140        150        160        170        180 
VRLYHYDAVE VLNKEIVDGA LDEIRIYFPD PWHKKRHHKR RLIQPLFATL LVRKLRVGGC 

       190        200        210        220        230        240 
LHMATDWADY AEQMWDVLDA TPGLVNRAGL RGQVPCPDWR VQTRFERRGQ NLGHRVWNLL 


YDRV 

« Hide

References

[1]"Whole genome sequences of two Xylella fastidiosa strains (M12 and M23) causing almond leaf scorch disease in California."
Chen J., Xie G., Han S., Chertkov O., Sims D., Civerolo E.L.
J. Bacteriol. 192:4534-4534(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: M23.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001011 Genomic DNA. Translation: ACB93381.1.
RefSeqYP_001830655.1. NC_010577.1.

3D structure databases

ProteinModelPortalB2I9C6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405441.XfasM23_1982.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACB93381; ACB93381; XfasM23_1982.
GeneID6202751.
KEGGxfn:XfasM23_1982.
PATRIC24146604. VBIXylFas85937_2463.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000073968.
KOK03439.
OMAHSTLEMA.
OrthoDBEOG6K6VBC.

Enzyme and pathway databases

BioCycXFAS405441:GJJI-2024-MONOMER.
UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_XYLF2
AccessionPrimary (citable) accession number: B2I9C6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways