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B2I730

- PNP_XYLF2

UniProt

B2I730 - PNP_XYLF2

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, XfasM23_0183
Organism
Xylella fastidiosa (strain M23)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi487 – 4871Magnesium By similarity
Metal bindingi493 – 4931Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciXFAS405441:GJJI-199-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:XfasM23_0183
OrganismiXylella fastidiosa (strain M23)
Taxonomic identifieri405441 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella
ProteomesiUP000001698: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 700700Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_1000192509Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Protein-protein interaction databases

STRINGi405441.XfasM23_0183.

Structurei

3D structure databases

ProteinModelPortaliB2I730.
SMRiB2I730. Positions 618-691.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini554 – 61360KH
Add
BLAST
Domaini623 – 69169S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2I730-1 [UniParc]FASTAAdd to Basket

« Hide

MAKITKTFQY GKHTVTLETG EVARQASGAV IVKMDDTVLL VTVVAAKTAR    50
EGQDFFPLTV DYQEKFYAGG RIPGSFFKRE GRATEKETLI SRLIDRPIRP 100
LFPEDYKNEV QIIAMVMSLD PEIDGDIPAM IGASAALSLA GIPFKGPIGA 150
AKVGYNDGQY ILNPTVSELK KSQLELVVAG TANAVLMVES EAALLSEEVM 200
LGAVIFGHRE MQKVIQVIDS LTAEACTQPS DWVPPAKNDA LVIALKKVIG 250
GRLSDAFHIR DKLQRRDAIA AVKDDVIQQL AGRLEVEGWN LAELLKEFGE 300
LEYRTMRDAL LDTKVRIDGR SLEAVRPITV KVGVLPRVHG SGLFTRGETQ 350
AIVVTTLGTA RDGQVIDAVT GEYKENFLFH YNFPPYSVGE CGRFGVPKRR 400
EIGHGRLARR GVLAVMPSLD EFPYTIRVVS EITESNGSSS MASVCGSSLA 450
LMDAGVPVKA PVAGIAMGLV KEGERFVVLS DILGDEDHLG DMDFKVAGTS 500
EGVSALQMDI KIEGITEEIM RQALQQAKVG RLHILGEMDK ALAAPRAELS 550
DYAPRLLTIK IHPDKIRDVI GKGGSTIQAI TKETGTQIDI QDDGTIVIAS 600
VNNAAAREAK RRIEQITSDV EPGRIYEGKV AKIMDFGAFV TILPGKDGLV 650
HVSQISSERV ERVGDKLKEG DVVRVKVLEV DKQGRIRLSI KAVEEEVASI 700
Length:700
Mass (Da):76,019
Last modified:June 10, 2008 - v1
Checksum:iE726D9F7ACBA1C6F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001011 Genomic DNA. Translation: ACB91640.1.
RefSeqiWP_012382430.1. NC_010577.1.
YP_001828914.1. NC_010577.1.

Genome annotation databases

EnsemblBacteriaiACB91640; ACB91640; XfasM23_0183.
GeneIDi6202555.
KEGGixfn:XfasM23_0183.
PATRICi24142115. VBIXylFas85937_0248.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001011 Genomic DNA. Translation: ACB91640.1 .
RefSeqi WP_012382430.1. NC_010577.1.
YP_001828914.1. NC_010577.1.

3D structure databases

ProteinModelPortali B2I730.
SMRi B2I730. Positions 618-691.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405441.XfasM23_0183.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACB91640 ; ACB91640 ; XfasM23_0183 .
GeneIDi 6202555.
KEGGi xfn:XfasM23_0183.
PATRICi 24142115. VBIXylFas85937_0248.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci XFAS405441:GJJI-199-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole genome sequences of two Xylella fastidiosa strains (M12 and M23) causing almond leaf scorch disease in California."
    Chen J., Xie G., Han S., Chertkov O., Sims D., Civerolo E.L.
    J. Bacteriol. 192:4534-4534(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: M23.

Entry informationi

Entry nameiPNP_XYLF2
AccessioniPrimary (citable) accession number: B2I730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: September 3, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi