B2I1V6 (MURD_ACIBC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	ACICU_00268

Organism

Acinetobacter baumannii (strain ACICU) [Complete proteome] [HAMAP]

Taxonomic identifier

405416 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_1000130821
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	B2I1V6 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: C581C04AA2FE2D9F	FASTA	448	48,009
	10 20 30 40 50 60 MLIQRGGLKV VAGLGISGVS AVNFLHEQGY QVAVTDSRPT PPGHDQIPAG VKTSFGQLDQ 70 80 90 100 110 120 ELLLQAEEII LSPGLAPQLP EIQAAIAKGI SVVGDIQLLR RATDVPIVAI TGSNAKSTVT 130 140 150 160 170 180 TLIGLMAKDA GKKVAVGGNL GRPALDLLKD QPELLVLELS SFQLETTSHL NAEVAVVLNM 190 200 210 220 230 240 SEDHLDRHGN MLGYHQAKHR IFQGAKKVVF NRDDALSRPL VPDTTPMQSF GLNAPDLNQY 250 260 270 280 290 300 GVLRDADGTL WLARGLQRLI KSSDLYIQGM HNVANALACL ALGEAIGLPM ESMLETLKRF 310 320 330 340 350 360 KGLEHRCEYV KTVRDVRYYN DSKGTNVGAT LAAIDGLGAA IEVKKGKVAL ILGGQGKGQD 370 380 390 400 410 420 FSPLRSSIEK YAKVVVLIGE DAPVIEQAIQ GATKILHAAT LKEAVELCQR ETQAEDVVLL 430 440 SPACASFDMF KSYNDRGQQF VACVNSLV

References

[1]

"Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group." Iacono M., Villa L., Fortini D., Bordoni R., Imperi F., Bonnal R.J., Sicheritz-Ponten T., De Bellis G., Visca P., Cassone A., Carattoli A. Antimicrob. Agents Chemother. 52:2616-2625(2008) [PubMed: 18411315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ACICU.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000863 Genomic DNA. Translation: ACC55580.1.
RefSeq	YP_001844927.1. NC_010611.1.
3D structure databases
ProteinModelPortal	B2I1V6.
ModBase	Search...
Protein-protein interaction databases
STRING	B2I1V6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6234698.
GenomeReviews	Gene locus ACICU_00268 in contig CP000863_GR.
KEGG	abc:ACICU_00268.
PATRIC	20709294. VBIAciBau103538_0367.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	PRK03803.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_ACIBC

Accession

Primary (citable) accession number: B2I1V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program