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B2I1J9 (PYRE_ACIBC) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Orotate phosphoribosyltransferase
Short name=OPRT
Short name=OPRTase
EC=2.4.2.10
Gene names
Name:pyrE
Ordered Locus Names:ACICU_03538
OrganismAcinetobacter baumannii (strain ACICU) [Complete proteome] [HAMAP]
Taxonomic identifier405416 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length216 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) By similarity. HAMAP MF_01208

Catalytic activity

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_01208

Cofactor

Magnesium By similarity. HAMAP MF_01208

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. HAMAP MF_01208

Subunit structure

Homodimer By similarity. HAMAP MF_01208

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   LigandMagnesium
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processnucleoside metabolic process

Inferred from electronic annotation. Source: InterPro

pyrimidine nucleotide biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionorotate phosphoribosyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 216216Orotate phosphoribosyltransferase HAMAP MF_01208
PRO_1000138755

Regions

Region38 – 392Orotate binding By similarity
Region75 – 7625-phosphoribose 1-diphosphate binding By similarity
Region128 – 13695-phosphoribose 1-diphosphate binding By similarity

Sites

Binding site3015-phosphoribose 1-diphosphate By similarity
Binding site10215-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10315-phosphoribose 1-diphosphate By similarity
Binding site10615-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site10815-phosphoribose 1-diphosphate; shared with dimeric partner By similarity
Binding site1321Orotate By similarity
Binding site1601Orotate By similarity

Sequences

Sequence LengthMass (Da)Tools
B2I1J9 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 84CB4E174146AFEA

FASTA21623,335
        10         20         30         40         50         60 
MTTPVSFHPQ AFIELALSRG VLKFGEFTLK SGRVSPYFFN AGLLNDGEAL SLLAQGYADK 

        70         80         90        100        110        120 
LTQCENVDVI FGPAYKGIPF VAATAVALSQ THNKSVPWGF NRKEAKDHGE GGVLVGAAVE 

       130        140        150        160        170        180 
GKKVWIIDDV ITAGTAIREV VTILKNAGAT IAGVLVALDR QERGQGELSA IQEVQKELEI 

       190        200        210 
PVHALITMKD LMDYLEAKGE KEALANMQAY REKYGI

« Hide

References

[1]"Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group."
Iacono M., Villa L., Fortini D., Bordoni R., Imperi F., Bonnal R.J., Sicheritz-Ponten T., De Bellis G., Visca P., Cassone A., Carattoli A.
Antimicrob. Agents Chemother. 52:2616-2625(2008) [PubMed: 18411315] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ACICU.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000863 Genomic DNA. Translation: ACC58847.1.
RefSeqYP_001848194.1. NC_010611.1.

3D structure databases

ProteinModelPortalB2I1J9.
ModBaseSearch...

Protein-protein interaction databases

STRINGB2I1J9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6234671.
GenomeReviewsGene locus ACICU_03538 in contig CP000863_GR.
KEGGabc:ACICU_03538.
PATRIC20715819. VBIAciBau103538_3558.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG404341.
OMAFAFNRKE.
ProtClustDBPRK00455.

Family and domain databases

HAMAPMF_01208. PyrE.
[Tree]
InterProIPR004467. Or_phspho_trans_clade-1.
IPR023031. Orotate_PribosylTferase.
IPR000836. PRibTrfase.
[Graphical view]
KOK00762.
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
TIGRFAMsTIGR00336. PyrE. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRE_ACIBC
AccessionPrimary (citable) accession number: B2I1J9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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