Reviewed, UniProtKB/Swiss-Prot B2HZV3 (TRMD_ACIBC)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	ACICU_03361

Organism

Acinetobacter baumannii (strain ACICU) [Complete proteome] [HAMAP]

Taxonomic identifier

405416 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_1000130120
Regions
	Region	137 – 142	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	117	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2HZV3-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 567F84D5112A2330	FASTA	246	27,881
	10 20 30 40 50 60 MFFAVITLFP EMFEAITAYG ISGRAAKRDI VQVTCINPRD FAEGNYRRVD ERPFGGGPGM 70 80 90 100 110 120 VMMAEPLAKA INHAKQLASQ AGCVHVPVVY MSPQGKTLNE QAVQQFVDYD GLIVLCGRYE 130 140 150 160 170 180 GVDERLIQHY VDQEWSIGDY VLSGGELPAM VLLDSIIRRL PNVMSDEQSA IQDSFVDGLL 190 200 210 220 230 240 DCPQYTKPDQ FEGLDVPEIL KSGHHANIEK WRFLQRYQRT LERRPELIEQ VTLTKQQKKW LSDEQG

References

[1]

"Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group." Iacono M., Villa L., Fortini D., Bordoni R., Imperi F., Bonnal R.J., Sicheritz-Ponten T., De Bellis G., Visca P., Cassone A., Carattoli A. Antimicrob. Agents Chemother. 52:2616-2625(2008) [PubMed: 18411315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000863 Genomic DNA. Translation: ACC58671.1.
RefSeq	YP_001848018.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6235741.
GenomeReviews	Gene locus ACICU_03361 in contig CP000863_GR.
KEGG	abc:ACICU_03361.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HRSVDDT.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_ACIBC

Accession

Primary (citable) accession number: B2HZV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)