Reviewed, UniProtKB/Swiss-Prot B2HZ52 (MDH_ACIBC)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase
    EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	ACICU_03222

Organism

Acinetobacter baumannii (strain ACICU) [Complete proteome] [HAMAP]

Taxonomic identifier

405416 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP MF_01517
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Malate dehydrogenase HAMAP MF_01517		PRO_1000191605
Regions
	Nucleotide binding	11 – 17	7	NAD By similarity
	Nucleotide binding	131 – 133	3	NAD By similarity
Sites
	Active site	189	1	Proton acceptor By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	107	1	NAD By similarity
	Binding site	114	1	NAD By similarity
	Binding site	133	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2HZ52-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 4A3ABDD04F615F6E	FASTA	328	35,323
	10 20 30 40 50 60 MKQPVRVAVT GAAGQIGYSL LFRIASGEML GKDQPVILQL LEVPVEKAQQ ALKGVMMELD 70 80 90 100 110 120 DCAFPLLAGM IGTDDPKVAF KDADYALLVG SRPRGPGMER ADLLKVNGEI FIGQGQALNE 130 140 150 160 170 180 VASRDVKVLV VGNPANTNAY IAMKSAPDLP AKNFTAMLRL DHNRALTQVA QKAGVAVADI 190 200 210 220 230 240 EKLTVWGNHS PTMYADYRFA TANGESLKDK INDPAWNKDV FLPTVGKRGA AIIEARGLSS 250 260 270 280 290 300 AASAANAAID HMRDWALGTN GKWVTMGVPS DGSYGIPEGV MFGFPVTTEN GEYKIVQGLE 310 320 IDEFSRERIN FTLNELEEER AAIADMVK

References

[1]

"Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group." Iacono M., Villa L., Fortini D., Bordoni R., Imperi F., Bonnal R.J., Sicheritz-Ponten T., De Bellis G., Visca P., Cassone A., Carattoli A. Antimicrob. Agents Chemother. 52:2616-2625(2008) [PubMed: 18411315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000863 Genomic DNA. Translation: ACC58534.1.
RefSeq	YP_001847881.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6234431.
GenomeReviews	Gene locus ACICU_03222 in contig CP000863_GR.
KEGG	abc:ACICU_03222.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MIIWGNH.
Family and domain databases
HAMAP	MF_01517. [Tree]
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
PROSITE	PS00068. MDH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDH_ACIBC

Accession

Primary (citable) accession number: B2HZ52

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)