B2HV28 (DUT_ACIBC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	ACICU_00837

Organism

Acinetobacter baumannii (strain ACICU) [Complete proteome] [HAMAP]

Taxonomic identifier

405416 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex › Acinetobacter baumannii

Protein attributes

Sequence length	150 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 150	150	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116		PRO_1000094935
Regions
	Region	69 – 71	3	Substrate binding By similarity
	Region	86 – 88	3	Substrate binding By similarity
Sites
	Binding site	82	1	Substrate By similarity
	Binding site	96	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2HV28 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: F3EBE361AA5BE853	FASTA	150	16,371
	10 20 30 40 50 60 MKVQVKLLDP RLGKEWPLPS YATAGSAGLD LRACLDEAIE IEPGQTVLVK TGMAIYIHDV 70 80 90 100 110 120 NFAGLILPRS GLGHKHGIVL GNLVGLIDSD YQGELMVSVW NRGQTTFRLE PGERLAQYVL 130 140 150 VPVVQAEFEQ VEEFEETLRG AGGFGHTGKQ

References

[1]

"Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group." Iacono M., Villa L., Fortini D., Bordoni R., Imperi F., Bonnal R.J., Sicheritz-Ponten T., De Bellis G., Visca P., Cassone A., Carattoli A. Antimicrob. Agents Chemother. 52:2616-2625(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ACICU.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000863 Genomic DNA. Translation: ACC56149.1.
RefSeq	YP_001845496.1. NC_010611.1.
3D structure databases
ProteinModelPortal	B2HV28.
SMR	B2HV28. Positions 2-137.
ModBase	Search...
Protein-protein interaction databases
STRING	405416.ACICU_00837.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACC56149; ACC56149; ACICU_00837.
GeneID	6236937.
KEGG	abc:ACICU_00837.
PATRIC	20710473. VBIAciBau103538_0934.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HOG000028968.
KO	K01520.
OMA	CLINLSN.
ProtClustDB	PRK00601.
Enzyme and pathway databases
BioCyc	ABAU405416:GI27-838-MONOMER.
UniPathway	UPA00610; UER00666.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact.
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_ACIBC

Accession

Primary (citable) accession number: B2HV28

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program