B2HUM5 (ISPE_ACIBC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	ACICU_00788

Organism

Acinetobacter baumannii (strain ACICU) [Complete proteome] [HAMAP]

Taxonomic identifier

405416 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter › Acinetobacter calcoaceticus/baumannii complex

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092054
Regions
	Nucleotide binding	91 – 101	11	ATP Potential
Sites
	Active site	9	1	By similarity
	Active site	133	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2HUM5 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 95BA6E3630E9F1AF	FASTA	277	31,067
	10 20 30 40 50 60 MIRVPSPAKL NLFLHITGRR ENGYHELQTI FQLIDLYDWM TFTPISEDEI QIEGLGEVQL 70 80 90 100 110 120 EQNLIYRAAQ ILRPHAQNPC GLHIKIEKNI PMGAGLGGGS SNAATTLIVL NQLWQCGLTE 130 140 150 160 170 180 EQLAQFGVKL GADVPIFIYG LNAWAEGIGE HLSFIDLDQK QFIVLKPDCF ISTQLLFSQK 190 200 210 220 230 240 TLTRDSKPTT FCAYQLEPSN FGNNFEPLAR ELYPEVEEAM QYLDQFGHAK LTGTGACVFA 250 260 270 EVTDEMNVDD ILKHAPCKAY LVHSLKESPL RHFKVAS

References

[1]

"Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group." Iacono M., Villa L., Fortini D., Bordoni R., Imperi F., Bonnal R.J., Sicheritz-Ponten T., De Bellis G., Visca P., Cassone A., Carattoli A. Antimicrob. Agents Chemother. 52:2616-2625(2008) [PubMed: 18411315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ACICU.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000863 Genomic DNA. Translation: ACC56100.1.
RefSeq	YP_001845447.1. NC_010611.1.
3D structure databases
ProteinModelPortal	B2HUM5.
ModBase	Search...
Protein-protein interaction databases
STRING	B2HUM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6233999.
GenomeReviews	Gene locus ACICU_00788 in contig CP000863_GR.
KEGG	abc:ACICU_00788.
PATRIC	20710371. VBIAciBau103538_0885.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	LNLFLHV.
ProtClustDB	PRK00343.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_ACIBC

Accession

Primary (citable) accession number: B2HUM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program