Reviewed, UniProtKB/Swiss-Prot B2HU41 (NUOA_ACIBC)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit A
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit A
    NDH-1 subunit A
    NUO1

Gene names

Name:	nuoA
Ordered Locus Names:	ACICU_00709

Organism

Acinetobacter baumannii (strain ACICU) [Complete proteome] [HAMAP]

Taxonomic identifier

405416 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Acinetobacter

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01394
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity. HAMAP MF_01394
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_01394.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 183	183	NADH-quinone oxidoreductase subunit A HAMAP MF_01394		PRO_0000362612
Regions
	Transmembrane	11 – 31	21	Potential
	Transmembrane	63 – 83	21	Potential
	Transmembrane	98 – 118	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	B2HU41-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: E0D568F82F867D31	FASTA	183	19,980
	10 20 30 40 50 60 MSAITPYDWA IIAFVIGVTF LCVFMLTVPL LLGGKSWGRA KQEQFESGVV SAGGARIRLS 70 80 90 100 110 120 AKFYLVAIFF VVFDLEALYL YAWSTSVREV GWLGYTTVVI FVVDLLIALV YAFSVGALSW 130 140 150 160 170 180 APADRRKLAG EKIKVGSPTM NIAEITRFNS IEELVTDPTG QIPAQSSGRV KSKTTPALSS EKE

References

[1]

"Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group." Iacono M., Villa L., Fortini D., Bordoni R., Imperi F., Bonnal R.J., Sicheritz-Ponten T., De Bellis G., Visca P., Cassone A., Carattoli A. Antimicrob. Agents Chemother. 52:2616-2625(2008) [PubMed: 18411315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000863 Genomic DNA. Translation: ACC56021.1.
RefSeq	YP_001845368.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6234357.
GenomeReviews	Gene locus ACICU_00709 in contig CP000863_GR.
KEGG	abc:ACICU_00709.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG605540.
OMA	ALYLYAY.
Family and domain databases
HAMAP	MF_01394. NDH1_NuoA. [Tree]
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA_ACIBC

Accession

Primary (citable) accession number: B2HU41

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	June 10, 2008
Last modified:	January 19, 2010
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)