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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Acinetobacter baumannii (strain ACICU)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei639 – 6391ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciABAU405416:GI27-570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:ACICU_00549
OrganismiAcinetobacter baumannii (strain ACICU)
Taxonomic identifieri405416 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeAcinetobacterAcinetobacter calcoaceticus/baumannii complex
ProteomesiUP000008839: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 874874Leucine--tRNA ligasePRO_1000091279Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi405416.ACICU_00549.

Structurei

3D structure databases

ProteinModelPortaliB2HTC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi636 – 6405"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B2HTC4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTISHIDPEY QANTIEPSVQ QDWENRKVFK VADTVEGKHR YILSMFPYPS
60 70 80 90 100
GKLHMGHVRN YTIGDVISRF YRLKGETVLQ PMGWDAFGLP AENAAIAHKV
110 120 130 140 150
APAKWTFENI AYMRDQLKKL GLSVDWDREF ATCTPEYYHW EQWLFVQLYK
160 170 180 190 200
KGLIYRKLST VNWDPVDQTV LANEQVENGR GWRSGALVEK RDIPMYYFRI
210 220 230 240 250
TDYAQELLDD LDTLQDGWPQ QVLTMQRNWI GRSTGMEITF PSANTEIYAD
260 270 280 290 300
GLTVYTTRAD TLMGVTYVAV AAEHPLALKA AENNPELAAF IEECRMGSVA
310 320 330 340 350
EADLATAEKK GMATGLFVKH PVTGEELPVW IANYVLMSYG SGAVMAVPAH
360 370 380 390 400
DERDFEFANK FNLPIKQVID AKGADDADYS ATEWQEWYGS KEGKLVNSGE
410 420 430 440 450
FDGLEFQAAF DAFLAKLEPQ GLANSKVQFR LRDWGVSRQR YWGCPIPMIN
460 470 480 490 500
CDTCGQVTVP EDQLPVVLPT DVVPDGSGNP LNKMPEFYET KCPCCGGDAR
510 520 530 540 550
RETDTLDTFV ESSWYYARYA SPDFTGGMVK PEAAKNWLPV NQYIGGVEHA
560 570 580 590 600
ILHLLYARFF HKLMRDEGVV QGNEPFTNLL TQGMVLADTF YREAENGKKT
610 620 630 640 650
WFNPADIELE RDEKGRIISA KYSGDGQEVI IGGQEKMSKS KNNGIDPQAI
660 670 680 690 700
IDQYGADTAR VFMMFAAPPD QSLEWSDAGV EGANRFLKRV WRLVASFLEK
710 720 730 740 750
GNSATAIDKA NLSKDAQDLR RKTHETIQKV SDDIERRHAF NTAIAALMEL
760 770 780 790 800
LNASNKFEAK DDNDVAVERE AITTLLTLLA PFAPHLSQTL LAQFGTDLTE
810 820 830 840 850
ATFPEVDASA LTRNTQTIVV QVNGKLRGKL EVSVDISKDE LLAQAKALPE
860 870
VQQFLTGPTK KEIVVPNKLV NLVV
Length:874
Mass (Da):98,080
Last modified:June 10, 2008 - v1
Checksum:i001491487750CA03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000863 Genomic DNA. Translation: ACC55861.1.
RefSeqiYP_001845208.1. NC_010611.1.

Genome annotation databases

EnsemblBacteriaiACC55861; ACC55861; ACICU_00549.
GeneIDi6236773.
KEGGiabc:ACICU_00549.
PATRICi20709877. VBIAciBau103538_0648.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000863 Genomic DNA. Translation: ACC55861.1.
RefSeqiYP_001845208.1. NC_010611.1.

3D structure databases

ProteinModelPortaliB2HTC4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405416.ACICU_00549.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC55861; ACC55861; ACICU_00549.
GeneIDi6236773.
KEGGiabc:ACICU_00549.
PATRICi20709877. VBIAciBau103538_0648.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciABAU405416:GI27-570-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole-genome pyrosequencing of an epidemic multidrug-resistant Acinetobacter baumannii strain belonging to the European clone II group."
    Iacono M., Villa L., Fortini D., Bordoni R., Imperi F., Bonnal R.J., Sicheritz-Ponten T., De Bellis G., Visca P., Cassone A., Carattoli A.
    Antimicrob. Agents Chemother. 52:2616-2625(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ACICU.

Entry informationi

Entry nameiSYL_ACIBC
AccessioniPrimary (citable) accession number: B2HTC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: June 10, 2008
Last modified: January 7, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.