false2008-06-102024-03-2792B2HT54_MYCMMInsights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis.Stinear T.P.Seemann T.Harrison P.F.Jenkin G.A.Davies J.K.Johnson P.D.Abdellah Z.Arrowsmith C.Chillingworth T.Churcher C.Clarke K.Cronin A.Davis P.Goodhead I.Holroyd N.Jagels K.Lord A.Moule S.Mungall K.Norbertczak H.Quail M.A.Rabbinowitsch E.Walker D.White B.Whitehead S.Small P.L.Brosch R.Ramakrishnan L.Fischbach M.A.Parkhill J.Cole S.T.doi:10.1101/gr.075069.1072008Genome Res.18729-741NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]ATCC BAA-535 / MIncreasing the structural coverage of tuberculosis drug targets.Baugh L.Phan I.Begley D.W.Clifton M.C.Armour B.Dranow D.M.Taylor B.M.Muruthi M.M.Abendroth J.Fairman J.W.Fox D.Dieterich S.H.Staker B.L.Gardberg A.S.Choi R.Hewitt S.N.Napuli A.J.Myers J.Barrett L.K.Zhang Y.Ferrell M.Mundt E.Thompkins K.Tran N.Lyons-Abbott S.Abramov A.Sekar A.Serbzhinskiy D.Lorimer D.Buchko G.W.Stacy R.Stewart L.J.Edwards T.E.Van Voorhis W.C.Myler P.J.doi:10.1016/j.tube.2014.12.0032015Tuberculosis95142-148X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 1-4741.85A/B/C/D=1-474BacteriaFumarase_classIIFumarase/aspartase (C-terminal domain)Fumarase/aspartase (Central domain)Fumarase/aspartase (N-terminal domain)FumaraseCFum_hydIIFumarase/histidase_NFumarase_C_CFumarate_lyase_CSFumarate_lyase_famFumarate_lyase_NL-Aspartase-likeASPARTATEAMMONIA/ARGININOSUCCINATE/ADENYLOSUCCINATE LYASEFUMARATE HYDRATASE CLASS IIFumaraseC_CLyase_1FUMRATELYASEL-aspartase-likeFUMARATE_LYASESFumarate hydratase class IIFumarase C4.2.1.2Aerobic fumaraseIron-independent fumarasefumfumCMMAR_4368Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.Homotetramer.There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.Fumarate lyase N-terminal23347Fumarase C C-terminal413469Proton donor/acceptor192323109111in site B133136143145191324329331Important for catalytic activity336substrate2008-06-10150192cc9289e5b7e39064b36c093bcf934046MAHSAHDDADNDTEYRIEHDTMGEVRVPAKALWRAQTQRAVENFPISGRGLERAQIRALGLLKGACAQVNMDLGLLAPEKAEAIIAAAAEIADGQHDDQFPIDVFQTGSGTSSNMNTNEVIASIAGANGVAVHPNDDVNMSQSSNDTFPTATHIAATEAAVSHLIPALEILQDALATKALEWQSVVKSGRTHLMDAVPVTLGQEFSGYARQIEAGIERVRATLPRLGELAIGGTAVGTGLNAPEGFGVKVVSVLVSQTGLPQLRTAANSFEAQAARDGLVEASGALRTIAVSLTKIANDIRWMGSGPLTGLAEIQLPDLQPGSSIMPGKVNPVLPEAVTQVAAQVIGNDAAVAWGGANGAFELNVYIPMMARNILESFTLLTNVSKLFAQRCIAGLTANAEHLRELAESSPSIVTPLNSAIGYEEAAAVAKQALKERKTIRQTVIDRGLIGDKLSLEELDRRLDVLAMAKVEATDD