Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fumarate hydratase class II

Gene

fum

Organism
Mycobacterium marinum (strain ATCC BAA-535 / M)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fum)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei191SubstrateUniRule annotation1
Active sitei192Proton donor/acceptorUniRule annotation1
Active sitei323UniRule annotation1
Binding sitei324SubstrateUniRule annotation1
Sitei336Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotation
Biological processTricarboxylic acid cycleUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumImported
Synonyms:fumCUniRule annotation
Ordered Locus Names:MMAR_4368Imported
OrganismiMycobacterium marinum (strain ATCC BAA-535 / M)Imported
Taxonomic identifieri216594 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000001190 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi216594.MMAR_4368.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QBPX-ray1.85A/B/C/D1-474[»]
ProteinModelPortaliB2HT54.
SMRiB2HT54.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiB2HT54.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 347Lyase_1InterPro annotationAdd BLAST325
Domaini413 – 469FumaraseC_CInterPro annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 111Substrate bindingUniRule annotation3
Regioni133 – 136Substrate binding (B site)UniRule annotation4
Regioni143 – 145Substrate bindingUniRule annotation3
Regioni329 – 331Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061737.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

B2HT54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHSAHDDAD NDTEYRIEHD TMGEVRVPAK ALWRAQTQRA VENFPISGRG
60 70 80 90 100
LERAQIRALG LLKGACAQVN MDLGLLAPEK AEAIIAAAAE IADGQHDDQF
110 120 130 140 150
PIDVFQTGSG TSSNMNTNEV IASIAGANGV AVHPNDDVNM SQSSNDTFPT
160 170 180 190 200
ATHIAATEAA VSHLIPALEI LQDALATKAL EWQSVVKSGR THLMDAVPVT
210 220 230 240 250
LGQEFSGYAR QIEAGIERVR ATLPRLGELA IGGTAVGTGL NAPEGFGVKV
260 270 280 290 300
VSVLVSQTGL PQLRTAANSF EAQAARDGLV EASGALRTIA VSLTKIANDI
310 320 330 340 350
RWMGSGPLTG LAEIQLPDLQ PGSSIMPGKV NPVLPEAVTQ VAAQVIGNDA
360 370 380 390 400
AVAWGGANGA FELNVYIPMM ARNILESFTL LTNVSKLFAQ RCIAGLTANA
410 420 430 440 450
EHLRELAESS PSIVTPLNSA IGYEEAAAVA KQALKERKTI RQTVIDRGLI
460 470
GDKLSLEELD RRLDVLAMAK VEATDD
Length:476
Mass (Da):50,192
Last modified:June 10, 2008 - v1
Checksum:iEA3DEA92B5FFD49F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA. Translation: ACC42775.1.
RefSeqiWP_012395931.1. NC_010612.1.

Genome annotation databases

EnsemblBacteriaiACC42775; ACC42775; MMAR_4368.
KEGGimmi:MMAR_4368.

Similar proteinsi

Entry informationi

Entry nameiB2HT54_MYCMM
AccessioniPrimary (citable) accession number: B2HT54
Entry historyiIntegrated into UniProtKB/TrEMBL: June 10, 2008
Last sequence update: June 10, 2008
Last modified: August 30, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported