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B2HT14 (B2HT14_MYCMM) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172
EC=2.1.1.14 HAMAP MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase HAMAP MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP MF_00172
Gene names
Name:metE HAMAP MF_00172 EMBL ACC42735.1
Ordered Locus Names:MMAR_4328
OrganismMycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]
Taxonomic identifier216594 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length763 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172 SAAS SAAS013215

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172 SAAS SAAS013215

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172 SAAS SAAS013215

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6501Zinc By similarity HAMAP MF_00172
Metal binding6521Zinc By similarity HAMAP MF_00172
Metal binding7351Zinc By similarity HAMAP MF_00172

Sequences

Sequence LengthMass (Da)Tools
B2HT14 [UniParc].

Last modified June 10, 2008. Version 1.
Checksum: 7C71CC41EDABCFF2

FASTA76382,111
        10         20         30         40         50         60 
MTQLVSQQPF FATITGSPRI GPRRELKRAT EGYWAGRTNR AELESVAATL RRDTWTDLAA 

        70         80         90        100        110        120 
AGLDSVPVNT FSYYDQMLDT AVLLGALPTR VAGICDDLDR YFAAARGNAE VAPLEMTKWF 

       130        140        150        160        170        180 
DTNYHYLVPE IGPDTRFGLH PDKVLAELKE AQELGIPARP VVIGPVTFLL LSKAVDGASA 

       190        200        210        220        230        240 
PIGRLEELMP IYSELLSLLA DSGAQWVQLD EPALVTDICP DAPALAERMY TALGSLANRP 

       250        260        270        280        290        300 
AIHVATYFGD PGAALGALAR TPIEAIGVDL VAGAHTAIAS IVAAPELADK TLVAGIVDGR 

       310        320        330        340        350        360 
NIWRTDLQAA LSRLATLLGS AASIAVSTSC STLHVPYSLE PETDLDASLR SWLAFGAEKV 

       370        380        390        400        410        420 
AEVAVLARAL REGREAVAAE IAASNEAVAS RKKDPRLHNG QVRARIHAIL ASGVHRGDAA 

       430        440        450        460        470        480 
ARRLSQEARL QLPPLPTTTI GSYPQTTAIR TARAALRADE IDEAEYARRM EKEIADVIAL 

       490        500        510        520        530        540 
QEKLGLDVLV HGEPERNDMV QYFAEQLDGF FATQNGWVQS YGSRCVRPPI LYGDVARSHP 

       550        560        570        580        590        600 
MTVDWISYAQ SLTAKPVKGM LTGPVTILAW SFVRDDQALP DTANQVALAI RDETVDLQSA 

       610        620        630        640        650        660 
GIAVIQVDEP ALRELLPLRR SEQDVYLRWA VAAFRLATSG VADSTQIHTH LCYSEFGEVI 

       670        680        690        700        710        720 
GAIADLDADV TSIEAARSHM EVLDDLNAIG FANSVGPGVY DIHSPRVPSA EEMADSLRAA 

       730        740        750        760 
LKAVPAGRLW ANPDCGLKTR NVDEVTASLQ NMVAAAQKVR PEA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000854 Genomic DNA. Translation: ACC42735.1.
RefSeqYP_001852590.1. NC_010612.1.

3D structure databases

ProteinModelPortalB2HT14.
ModBaseSearch...

Protein-protein interaction databases

STRINGB2HT14.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000031659; EBMYCP00000030099; EBMYCG00000031654.
GeneID6228610.
GenomeReviewsGene locus MMAR_4328 in contig CP000854_GR.
KEGGmmi:MMAR_4328.
PATRIC18069976. VBIMycMar75906_4654.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000017657.
HOGENOMHBG287495.
OMARNIWRAN.
ProtClustDBPRK05222.

Family and domain databases

HAMAPMF_00172. Meth_synth.
[Tree]
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
KOK00549.
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. Met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameB2HT14_MYCMM
AccessionPrimary (citable) accession number: B2HT14
Entry history
Integrated into UniProtKB/TrEMBL: June 10, 2008
Last sequence update: June 10, 2008
Last modified: December 14, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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