Reviewed, UniProtKB/Swiss-Prot B2HSY2 (MASZ_MYCMM)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	MMAR_2713

Organism

Mycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]

Taxonomic identifier

216594 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	Malate synthase G HAMAP MF_00641		PRO_1000130893
Sites
	Active site	339	1	Proton acceptor By similarity
	Active site	637	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B2HSY2-1 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: B1E03DE79642E7DC	FASTA	731	79,479
	10 20 30 40 50 60 MTDRVSAGNL RVARVLYDFV NNEALPGTDI DQDSFWAGVD KVVTDLTPQN QDLLKTRDDL 70 80 90 100 110 120 QAQIDKWHRH RVIEPLDPQA YREFLTEIGY LLPAPEDFTI TTSGVDDEIT TTAGPQLVVP 130 140 150 160 170 180 ILNARFALNA ANARWGSLYD ALYGTDVISE SDGAEKGRGY NKVRGDKVIA YARQFLDDSV 190 200 210 220 230 240 PLAGASYTDA TGFKVEDGQL VVSLADTSAA LADPGQFAGY TGTAENPKSI LLANHGLHIE 250 260 270 280 290 300 ILIDPESQIG ATDGAGVKDV ILESAITTIM DFEDSVAAVD ADDKVLGYRN WLGLNRGDLS 310 320 330 340 350 360 EDVTKDDKTF TRVLNTDRTY TAPHGGELTL PGRSLLFVRN VGHLMTNDAI VSDAEGAEGA 370 380 390 400 410 420 PVFEGIMDAL FTGLIAIHGL RSTDANGLLT NSRTGSIYIV KPKMHGPAEV AFTCELFSRV 430 440 450 460 470 480 EDVLGLPQGT MKVGIMDEER RTTLNLKACI KAAADRVVFI NTGFLDRTGD EIHTSMEAGP 490 500 510 520 530 540 MIRKGAMKNT AWIKAYEDAN VDTGLAAGFS GKAQIGKGMW AMTELMADMV EQKIAQPKAG 550 560 570 580 590 600 ATTAWVPSPT AATLHAMHYH KVDVFAVQKE LQGKTRTSVD ELLTIPLAKE LAWAPEEIRE 610 620 630 640 650 660 EVDNNCQSIL GYVVRWIDQG VGCSKVPDIH NVALMEDRAT LRISSQLLAN WLRHGVITSE 670 680 690 700 710 720 DARASLERMA PLVDKQNAGD PEYHAMAPNF DDSIAFLAAQ DLILSGAQQP NGYTEPILHR 730 RRRELKARAG A

References

[1]

"Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis." Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K., Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C., Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K., Lord A., Moule S. , Mungall K., Norbertczak H., Quail M.A., Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R., Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T. Genome Res. 18:729-741(2008) [PubMed: 18403782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000854 Genomic DNA. Translation: ACC41156.1.
RefSeq	YP_001851011.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6226980.
GenomeReviews	Gene locus MMAR_2713 in contig CP000854_GR.
KEGG	mmi:MMAR_2713.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PKMHGPD.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_MYCMM

Accession

Primary (citable) accession number: B2HSY2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	June 10, 2008
Last modified:	November 3, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)