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Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium marinum (strain ATCC BAA-535 / M)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei275Acetyl-CoAUniRule annotation1
Binding sitei312Acetyl-CoAUniRule annotation1
Active sitei339Proton acceptorUniRule annotation1
Binding sitei339GlyoxylateUniRule annotation1
Metal bindingi438MagnesiumUniRule annotation1
Binding sitei438GlyoxylateUniRule annotation1
Metal bindingi466MagnesiumUniRule annotation1
Binding sitei547Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei637Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:MMAR_2713
OrganismiMycobacterium marinum (strain ATCC BAA-535 / M)
Taxonomic identifieri216594 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000001190 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001308931 – 731Malate synthase GAdd BLAST731

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei623Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi216594.MMAR_2713.

Structurei

Secondary structure

1731
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 7Combined sources4
Beta strandi10 – 13Combined sources4
Helixi14 – 22Combined sources9
Helixi32 – 69Combined sources38
Beta strandi72 – 74Combined sources3
Helixi78 – 87Combined sources10
Helixi107 – 110Combined sources4
Beta strandi116 – 120Combined sources5
Helixi124 – 131Combined sources8
Helixi132 – 134Combined sources3
Beta strandi135 – 137Combined sources3
Helixi138 – 143Combined sources6
Turni151 – 153Combined sources3
Helixi162 – 179Combined sources18
Helixi187 – 189Combined sources3
Beta strandi192 – 196Combined sources5
Beta strandi199 – 203Combined sources5
Beta strandi205 – 209Combined sources5
Beta strandi211 – 213Combined sources3
Helixi214 – 216Combined sources3
Beta strandi217 – 222Combined sources6
Beta strandi224 – 234Combined sources11
Beta strandi237 – 243Combined sources7
Helixi250 – 252Combined sources3
Beta strandi257 – 263Combined sources7
Beta strandi267 – 273Combined sources7
Helixi281 – 296Combined sources16
Beta strandi301 – 304Combined sources4
Beta strandi309 – 312Combined sources4
Beta strandi318 – 321Combined sources4
Beta strandi325 – 330Combined sources6
Beta strandi335 – 339Combined sources5
Beta strandi346 – 352Combined sources7
Beta strandi358 – 363Combined sources6
Helixi364 – 376Combined sources13
Helixi377 – 380Combined sources4
Turni384 – 386Combined sources3
Beta strandi393 – 395Combined sources3
Beta strandi397 – 401Combined sources5
Helixi407 – 424Combined sources18
Beta strandi431 – 437Combined sources7
Helixi440 – 443Combined sources4
Helixi446 – 452Combined sources7
Turni453 – 456Combined sources4
Beta strandi457 – 462Combined sources6
Helixi464 – 474Combined sources11
Helixi476 – 478Combined sources3
Helixi484 – 489Combined sources6
Helixi491 – 506Combined sources16
Turni510 – 512Combined sources3
Beta strandi513 – 517Combined sources5
Helixi526 – 532Combined sources7
Helixi535 – 538Combined sources4
Beta strandi542 – 548Combined sources7
Helixi549 – 561Combined sources13
Helixi564 – 571Combined sources8
Helixi579 – 582Combined sources4
Helixi595 – 619Combined sources25
Beta strandi624 – 627Combined sources4
Beta strandi633 – 636Combined sources4
Helixi638 – 653Combined sources16
Helixi659 – 676Combined sources18
Turni677 – 679Combined sources3
Helixi690 – 692Combined sources3
Helixi694 – 704Combined sources11
Helixi706 – 708Combined sources3
Helixi710 – 712Combined sources3
Helixi715 – 729Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6AXEX-ray1.60A/B1-731[»]
SMRiB2HSY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni463 – 466Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3. Bacteria.
COG2225. LUCA.
HOGENOMiHOG000220740.
KOiK01638.
OMAiTEPVLHA.
OrthoDBiPOG091H05P0.

Family and domain databases

CDDicd00728. malate_synt_G. 1 hit.
Gene3Di2.170.170.11. 1 hit.
HAMAPiMF_00641. Malate_synth_G. 1 hit.
InterProiView protein in InterPro
IPR011076. Malate_synth-like_sf.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
PfamiView protein in Pfam
PF01274. Malate_synthase. 1 hit.
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

B2HSY2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRVSAGNL RVARVLYDFV NNEALPGTDI DQDSFWAGVD KVVTDLTPQN
60 70 80 90 100
QDLLKTRDDL QAQIDKWHRH RVIEPLDPQA YREFLTEIGY LLPAPEDFTI
110 120 130 140 150
TTSGVDDEIT TTAGPQLVVP ILNARFALNA ANARWGSLYD ALYGTDVISE
160 170 180 190 200
SDGAEKGRGY NKVRGDKVIA YARQFLDDSV PLAGASYTDA TGFKVEDGQL
210 220 230 240 250
VVSLADTSAA LADPGQFAGY TGTAENPKSI LLANHGLHIE ILIDPESQIG
260 270 280 290 300
ATDGAGVKDV ILESAITTIM DFEDSVAAVD ADDKVLGYRN WLGLNRGDLS
310 320 330 340 350
EDVTKDDKTF TRVLNTDRTY TAPHGGELTL PGRSLLFVRN VGHLMTNDAI
360 370 380 390 400
VSDAEGAEGA PVFEGIMDAL FTGLIAIHGL RSTDANGLLT NSRTGSIYIV
410 420 430 440 450
KPKMHGPAEV AFTCELFSRV EDVLGLPQGT MKVGIMDEER RTTLNLKACI
460 470 480 490 500
KAAADRVVFI NTGFLDRTGD EIHTSMEAGP MIRKGAMKNT AWIKAYEDAN
510 520 530 540 550
VDTGLAAGFS GKAQIGKGMW AMTELMADMV EQKIAQPKAG ATTAWVPSPT
560 570 580 590 600
AATLHAMHYH KVDVFAVQKE LQGKTRTSVD ELLTIPLAKE LAWAPEEIRE
610 620 630 640 650
EVDNNCQSIL GYVVRWIDQG VGCSKVPDIH NVALMEDRAT LRISSQLLAN
660 670 680 690 700
WLRHGVITSE DARASLERMA PLVDKQNAGD PEYHAMAPNF DDSIAFLAAQ
710 720 730
DLILSGAQQP NGYTEPILHR RRRELKARAG A
Length:731
Mass (Da):79,479
Last modified:June 10, 2008 - v1
Checksum:iB1E03DE79642E7DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA. Translation: ACC41156.1.
RefSeqiWP_012394427.1. NC_010612.1.

Genome annotation databases

EnsemblBacteriaiACC41156; ACC41156; MMAR_2713.
KEGGimmi:MMAR_2713.

Similar proteinsi

Entry informationi

Entry nameiMASZ_MYCMM
AccessioniPrimary (citable) accession number: B2HSY2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: March 28, 2018
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome