B2HRU9 (DER_MYCMM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase Der

Alternative name(s):
GTP-binding protein EngA

Gene names

Name:	der
Synonyms:	engA
Ordered Locus Names:	MMAR_2528

Organism

Mycobacterium marinum (strain ATCC BAA-535 / M) [Complete proteome] [HAMAP]

Taxonomic identifier

216594 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	GTPase that plays an essential role in the late steps of ribosome biogenesis By similarity. HAMAP MF_00195
Subunit structure	Associates with the 50S ribosomal subunit By similarity.
Sequence similarities	Belongs to the Era/MnmE GTP-binding protein family. Der subfamily. Contains 2 G (guanine nucleotide-binding) domains. Contains 1 KH-like domain.

Ontologies

Keywords
Biological process	Ribosome biogenesis
Domain	Repeat
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ribosome biogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	GTPase Der HAMAP MF_00195		PRO_1000099140
Regions
	Domain	41 – 146	106	G 1
	Domain	214 – 322	109	G 2
	Domain	376 – 459	84	KH-like
	Nucleotide binding	36 – 43	8	GTP 1 Potential
	Nucleotide binding	83 – 87	5	GTP 1 Potential
	Nucleotide binding	145 – 148	4	GTP 1 Potential
	Nucleotide binding	209 – 216	8	GTP 2 Potential
	Nucleotide binding	256 – 260	5	GTP 2 Potential
	Nucleotide binding	321 – 324	4	GTP 2 Potential

Sequences

	Sequence		Length	Mass (Da)
	B2HRU9 [UniParc]. Last modified June 10, 2008. Version 1. Checksum: 2269E4541E1F09E4	FASTA	469	50,871
	10 20 30 40 50 60 MTQDGTWSDE SDWELDDADL ADLAEAAPAP VLAIVGRPNV GKSTLVNRIL GRREAVVQDI 70 80 90 100 110 120 PGVTRDRVSY DALWNGRRFV VQDTGGWEPD AKGLQQLVAE QASVAMRTAD AVVLVVDATV 130 140 150 160 170 180 GATTADEAAA RILLRSGKPV FLAANKVDSD KAEADAATLW SMGLGEPHAI SAMHGRGVAD 190 200 210 220 230 240 LLDTVLKKLP EVAESVSAGG GPRRVALVGK PNVGKSSLLN KLAGDERSVV HDVAGTTVDP 250 260 270 280 290 300 VDSLIELGGK VWRFVDTAGL RRKVGQASGH EFYASVRTRG AIDAAEVVVL LIDASQPLTE 310 320 330 340 350 360 QDLRVLSMVI EAGRAVVLAY NKWDLVDEDR RDLLDREIDR ELVQIRWAQR VNISAKTGRA 370 380 390 400 410 420 VQKLVPAMET ALASWDTRIP TGPLNSWIKE VVAATPPPVR GGKQPRILFA TQATARPPTF 430 440 450 460 VLFTTGFLEA GYRRFLERRL RETFGFEGSP IRINVRVREK RGAKRPGRR

References

[1]

"Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis." Stinear T.P., Seemann T., Harrison P.F., Jenkin G.A., Davies J.K., Johnson P.D., Abdellah Z., Arrowsmith C., Chillingworth T., Churcher C., Clarke K., Cronin A., Davis P., Goodhead I., Holroyd N., Jagels K., Lord A., Moule S. , Mungall K., Norbertczak H., Quail M.A., Rabbinowitsch E., Walker D., White B., Whitehead S., Small P.L., Brosch R., Ramakrishnan L., Fischbach M.A., Parkhill J., Cole S.T. Genome Res. 18:729-741(2008) [PubMed: 18403782] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-535 / M.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000854 Genomic DNA. Translation: ACC40979.1.
RefSeq	YP_001850834.1. NC_010612.1.
3D structure databases
ProteinModelPortal	B2HRU9.
ModBase	Search...
Protein-protein interaction databases
STRING	B2HRU9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000031955; EBMYCP00000030395; EBMYCG00000031950.
GeneID	6226795.
GenomeReviews	Gene locus MMAR_2528 in contig CP000854_GR.
KEGG	mmi:MMAR_2528.
PATRIC	18066021. VBIMycMar75906_2691.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016947.
HOGENOM	HBG592135.
OMA	LAVNKCE.
ProtClustDB	PRK03003.
Family and domain databases
HAMAP	MF_00195. GTPase_Der. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR016484. GTP-bd_EngA. IPR006073. GTP_binding_domain. IPR015946. KH_dom-like_a/b. IPR005225. Small_GTP-bd_dom. [Graphical view]
Gene3D	G3DSA:3.30.300.20. KH_prok. 1 hit.
KO	K03977.
PANTHER	PTHR11649:SF5. PTHR11649:SF5. 1 hit.
Pfam	PF01926. MMR_HSR1. 2 hits. [Graphical view]
PIRSF	PIRSF006485. GTP-binding_EngA. 1 hit.
PRINTS	PR00326. GTP1OBG.
SMART	SM00382. AAA. 2 hits. [Graphical view]
TIGRFAMs	TIGR03594. GTPase_EngA. 1 hit. TIGR00231. Small_GTP. 2 hits.
ProtoNet	Search...

Entry information

Entry name

DER_MYCMM

Accession

Primary (citable) accession number: B2HRU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 10, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program