Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium marinum (strain ATCC BAA-535 / M)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 4311D-inositol 3-phosphateUniRule annotation
Binding sitei57 – 571UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei112 – 11211D-inositol 3-phosphateUniRule annotation
Binding sitei145 – 14511D-inositol 3-phosphateUniRule annotation
Binding sitei169 – 16911D-inositol 3-phosphateUniRule annotation
Binding sitei189 – 18911D-inositol 3-phosphateUniRule annotation
Binding sitei263 – 2631UDP-GlcNAcUniRule annotation
Binding sitei268 – 2681UDP-GlcNAcUniRule annotation
Binding sitei321 – 3211UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi330 – 3301Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi331 – 3311Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi333 – 3331Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei343 – 3431UDP-GlcNAcUniRule annotation
Binding sitei351 – 3511UDP-GlcNAcUniRule annotation
Metal bindingi357 – 3571MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMMAR216594:GJOB-813-MONOMER.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:MMAR_0812
OrganismiMycobacterium marinum (strain ATCC BAA-535 / M)
Taxonomic identifieri216594 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000001190 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 466466D-inositol 3-phosphate glycosyltransferasePRO_0000400136Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi216594.MMAR_0812.

Structurei

3D structure databases

ProteinModelPortaliB2HQV2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni49 – 502UDP-GlcNAc bindingUniRule annotation
Regioni54 – 5961D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

B2HQV2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPMRAGAGA AGESCKDDGV RPDDVSRLTA DAVVRRVALL AVHTSPLAQP
60 70 80 90 100
GTGDAGGMNV YVLQSALHLA KRGIEVEIFT RATASADPPV VRVAPGVLVR
110 120 130 140 150
NVVAGPFEGL DKYDLPTQLC AFAAGVLRAE AAHEPGHYDI VHSHYWLSGQ
160 170 180 190 200
VGWLARDRWA VPLVHTAHTL AAVKNAALAN GDAPEPPLRT VGEQQVVDEA
210 220 230 240 250
DRLIVNTDDE AKQLISIHRA DPARIDVVHP GVDLEVFRPG DRQQARTALG
260 270 280 290 300
LRPEEKVVAF VGRIQPLKAP DIVLRAVAKL PGVRIIVAGG PSGSGLASPD
310 320 330 340 350
GLAQLADELG IAERVTFLPP QSRTDLARVF HAVDLVAIPS YSESFGLVAV
360 370 380 390 400
EAQACGTPVV AAAVGGLPVA VRDGVSGTLV SGHDVDQWAA AIDGLLRSNA
410 420 430 440 450
GAQGALMSRA AAEHAATFSW ENTTDALLAS YRRAIGDFTA GRRRKVRDPV
460
AARKPRRWTA RRGVGA
Length:466
Mass (Da):49,055
Last modified:June 10, 2008 - v1
Checksum:iC7739A07D44D0816
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA. Translation: ACC39272.1.

Genome annotation databases

EnsemblBacteriaiACC39272; ACC39272; MMAR_0812.
KEGGimmi:MMAR_0812.
PATRICi18062357. VBIMycMar75906_0871.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA. Translation: ACC39272.1.

3D structure databases

ProteinModelPortaliB2HQV2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216594.MMAR_0812.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACC39272; ACC39272; MMAR_0812.
KEGGimmi:MMAR_0812.
PATRICi18062357. VBIMycMar75906_0871.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Enzyme and pathway databases

BioCyciMMAR216594:GJOB-813-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_MYCMM
AccessioniPrimary (citable) accession number: B2HQV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: June 10, 2008
Last modified: September 7, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.