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Protein

Thiazole synthase

Gene

thiG

Organism
Mycobacterium marinum (strain ATCC BAA-535 / M)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98Schiff-base intermediate with DXPUniRule annotation1
Binding sitei159DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processThiamine biosynthesis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:MMAR_0720
OrganismiMycobacterium marinum (strain ATCC BAA-535 / M)
Taxonomic identifieri216594 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000001190 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001968771 – 264Thiazole synthaseAdd BLAST264

Proteomic databases

PRIDEiB2HQ02

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi216594.MMAR_0720

Structurei

3D structure databases

ProteinModelPortaliB2HQ02
SMRiB2HQ02
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 186DXP bindingUniRule annotation2
Regioni207 – 208DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8 Bacteria
COG2022 LUCA
HOGENOMiHOG000248049
KOiK03149
OMAiAQYPSPA
OrthoDBiPOG091H01K9

Family and domain databases

CDDicd04728 ThiG, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00443 ThiG, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR033983 Thiazole_synthase_ThiG
IPR008867 ThiG
PANTHERiPTHR34266 PTHR34266, 1 hit
PfamiView protein in Pfam
PF05690 ThiG, 1 hit

Sequencei

Sequence statusi: Complete.

B2HQ02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVESKLTIAD RSFASRLIMG TGGASNLAVL QEALVASGTE LTTVAIRRVD
60 70 80 90 100
AEGGTGLLDL LNRLGITPLP NTAGCRSAAE AVLTAQLARE ALATNWVKLE
110 120 130 140 150
VIADERTLLP DAIELVRAAE QLVDDGFVVL PYTNDDPVLA RRLEDTGCAA
160 170 180 190 200
VMPLGSPIGT GLGITNPHNI EMIVASAGVP VVLDAGIGTA SDAALAMELG
210 220 230 240 250
CDAVLLATAV TRAADPAAMA AAMSAAVTAG YLARRAGRIP KRFWAQASSP
260
TLVTTQSPGA ESGN
Length:264
Mass (Da):26,969
Last modified:June 10, 2008 - v1
Checksum:i86E1875638E6E2A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000854 Genomic DNA Translation: ACC39179.1
RefSeqiWP_012392668.1, NC_010612.1

Genome annotation databases

EnsemblBacteriaiACC39179; ACC39179; MMAR_0720
KEGGimmi:MMAR_0720

Similar proteinsi

Entry informationi

Entry nameiTHIG_MYCMM
AccessioniPrimary (citable) accession number: B2HQ02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: June 10, 2008
Last modified: May 23, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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